Expression, Purification and Immunogenic Description of a Hepatitis C Virus Recombinant CoreE1E2 Protein Expressed by Yeast Pichia pastoris
| Author | Mehdi Fazlalipour | en |
| Author | Hossein Keyvani | en |
| Author | Seyed Hamid Reza Monavari | en |
| Author | Hamid Reza Mollaie | en |
| Orcid | Hossein Keyvani [0000-0001-9367-6955] | en |
| Issued Date | 2015-04-01 | en |
| Abstract | Background: Gradual development of a useful vaccine can be the main point in the control and eradication of Hepatitis C virus (HCV) infection. Hepatitis C Virus envelope glycoproteins are considered as the main HCV vaccine candidate. Objectives: In this study, the Pichia pastoris expression system was used to express a recombinant HCV CoreE1E2 protein, which consists of Core (269 nt-841nt) E1 (842 nt-1417nt) and E2 (1418 nt-2506nt). Materials and Methods: By a codon optimization technique based on the P. pastoris expression system, we could increase the rate of recombinant proteins. Moreover, the purified protein can efficiently induce anti-CoreE1E2 antibodies in rabbits, and also by developing a homemade Enzyme-Linked ELISA kit we can detect antibody of HCV Iranian patients with genotype 1a. Results: In our study, the virus-like particle of rCoreE1E2 with 70 nm size, was shown by Electron microscopy and proved the self-assembly in vitro in a yeast expression system. Conclusions: These findings of the present study indicate that the recombinant CoreE1E2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential HCV vaccine candidate. | en |
| DOI | https://doi.org/10.5812/jjm.8(4)2015.17157 | en |
| Keyword | Hepatitis C Virus (HCV) | en |
| Keyword | Virus Like Particles | en |
| Keyword | <i>Pichia pastoris</i> | en |
| Keyword | Recombinant Core-E1-E2 Glycoprotein | en |
| Publisher | Brieflands | en |
| Title | Expression, Purification and Immunogenic Description of a Hepatitis C Virus Recombinant CoreE1E2 Protein Expressed by Yeast Pichia pastoris | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 18883-pdf.pdf
- Size:
- 760.92 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF