Design and Evaluation of a Novel Anti-microbial Peptide from Cathelicidin-2: Selectively Active Against <i>Acinetobacter baumannii</i>
| Author | Fariba Fathi | en |
| Author | Maryam Ghobeh | en |
| Author | Farshad H Shirazi | en |
| Author | Maryam Tabarzad | en |
| Orcid | Fariba Fathi [0000-0002-3397-7083] | en |
| Orcid | Maryam Ghobeh [0000-0002-5038-5426] | en |
| Orcid | Farshad H Shirazi [0000-0003-1356-385X] | en |
| Orcid | Maryam Tabarzad [0000-0001-9923-0639] | en |
| Issued Date | 2023-12-31 | en |
| Abstract | Background: Infections caused by pathogenic microorganisms have increased the need for hospital care and have thus represented a public health problem and a significant financial burden. Classical treatments consisting of traditional antibiotics face several challenges today. Anti-microbial peptides (AMPs) are a conserved characteristic of the innate immune response among different animal species to defend against pathogenic microorganisms. Objectives: In this study, a new peptide sequence (mCHTL131-140) was designed using the in silico approach. Methods: Cathelicidin-2 (UniprotID: Q2IAL7) was used as a potential antimicrobial protein, and a novel 10 - 12 amino acids sequence AMP was designed using bioinformatics tools and the AMP databases. Then, the anti-bacterial, anti-biofilm, and anti-fungal properties of the peptide, as well as its hemolytic activity and cytotoxicity towards human fibroblast (HDF) cells, were investigated in vitro. Results: Online bioinformatics tools indicated that the peptide sequence could have anti-bacterial, anti-viral, anti-fungal, and anti-biofilm properties with little hemolytic properties. The experimental tests confirmed that mCHTL131-140 exhibited the best anti-bacterial properties against Acinetobacter baumannii and had fair anti-fungal properties. Besides, it did not cause red blood cell lysis and showed no cytotoxicity towards HDF cells. Conclusions: In general, the designed peptide can be considered a promising AMP to control hospital-acquired infections by A. baumannii. | en |
| DOI | https://doi.org/10.5812/ijpr-141920 | en |
| Keyword | Anti-microbial Peptides | en |
| Keyword | Design | en |
| Keyword | Hemolytic Activity | en |
| Keyword | <i>Acinetobacter baumannii</i> | en |
| Keyword | Anti-fungal | en |
| Publisher | Brieflands | en |
| Title | Design and Evaluation of a Novel Anti-microbial Peptide from Cathelicidin-2: Selectively Active Against <i>Acinetobacter baumannii</i> | en |
| Type | Research Article | en |