Relationship between synthesis of P21 and Cyclin D1 proteins and histomorphometric parameters of mice testicular tissue in presence of Aflatoxin G1

Abstract

Introduction: AFG1 after B1 is the most important aflatoxin found in beans and grains. Remarkably, aflatoxin cause a decrease in production and quality of sperm. In the current study, the effect of AFG1 on spermatogenesis, spermiogenicity, synthesis of P21 and cyclin D1 proteins in testicular tissue was investigated. Materials and Methods: 24 male albino mice were divided into 4 groups control group intraperitoneally received 0.2 ml corn oil and experimental groups received AFG1 (20 μg) for 7, 15, and 35 days, respectively. After 7, 15 and 35 days, the testicular tissue samples were taken, and after preparing tissue sections, hematoxylin and eosin (H;E) staining was performed. Histomorphometric parameters and the immunohistochemical analysis of proteins of P21 and cyclin D1 were investigated. Data analysis was carried out using one-way ANOVA at the significance level of p