Koomesh

Permanent URI for this collection

https://repository.brieflands.com/handle/123456789/60217

Browse

Now showing 1 - 20 of 1585

Status of zinc, copper and magnesium in serum and hair of insulin-dependent diabetes
(Brieflands, 1999-12-31) Hossein Nazari; Isa Normohammadi
Introduction. There is evidence that trace elemnets metabolism has changed in insulin-dependent diabetes melitus (IDDM) . In this study, status of zinc (Zn), copper (Cu) and magnesium (Mn) in serum and hair of IDDM patients was investigated. Materials and Methods. Two groups were studied: Patients with IDDM and healthy control. Zn, Cu, and Mn in serum and hair of these subjects were analyzed by atomic absorption. Results. In comparision with control group, in patiant group Cu of serum was sigmificantly decresed (P
Design and construction of fusion genes hspX and tb10.4 from Mycobacterium tuberculosis in a cloning vector
(Brieflands, 2017-03-31) Atieh Yaghoubi; Ehsan Aryan; Mohammad Derakhshan; Zahra Meshkat
Introduction: Tuberculosis (TB) is the most important infectious disease and is one of the most common causes of death in the world especially in developing countries. TB is caused by infection with Mycobacterium tuberculosis. Designing and construction new vaccines against Mycobacterium tuberculosis are the only effective way to prevent and control the disease. The aim of this study was to design and construct a cloning vector encoding hspX and tb10.4 fusion genes of Mycobacterium tuberculosis. Materials and Methods: At first, tb10.4 fragment was amplified by PCR method and it was digested with restriction enzymes and was cloned into the plasmid pcDNA3.1 +. Then, hspX fragment was amplified by PCR method and it was digested by HindIII and BamHI restriction enzymes. The recombinant plasmid pcDNA3.1 + / tb10.4 also digested with the same enzymes and hspX was subcloned into the recombinant vector. This construct was transformed into the Escherichia coli strain TOP10. The confirming the clones were performed by colony PCR, restriction enzyme digestion and sequencing methods. Results: PCR and enzymatic digestion products were run on the agarose gel and tb10.4 and hspX genes were observed 291bp and 435bp, respectively. In addition, results of DNA sequencing showed 100% homology with hspX and tb10.4 genes of Mycobacterium tuberculosis H37Rv strain recorded in GenBank. Conclusion: In this study, hspX and tb10.4 genes of Mycobacterium tuberculosis were cloned into pcDNA3.1 + vector correctly. This vector can use as a DNA vaccine to induce immune system responses in animal models in future studies.
Epidemiology of occupational accidents in Semnan (2002-2006)
(Brieflands, 2009-03-31) Ali ASghar Ghods; Fatemeh Alhani; Monireh Anosheh; Mehdi Kahoei
Introduction: Occupational injury is a major health problem in developing countries. The first step in trade Occupational injury problems is to identify pattern and characteristics. Thus, the aim of this study was to demonstrate status of work injuries admissions to trauma hospital in Semnan, as a main industrial city of Iran. Material and Methods: This descriptive study was performed based on the information collected from all injured patients admitted during to hospitals in Semnan during 2002-2006. Data analysis was performed using SPSS 11 Results: The results showed the incidence rate of occupational accidents was 3.8/1000 during years between 2002 and 2006. Occupational accidents were greater in young (20-24 years), male and married workers. Most of accidents had been happened between 7and 10 am. Finally, most related factors were personal factors (fatigue, multi-job, family problems, and using of medicines. These factors were responsible for 53.6% of all occupational accidents. Conclusion: It seems that constant safety education for the public and professional training for workers would reduce the incidence of occupational injuries. Prevention strategies and guidelines, particularly about personal factors that are implicated in injuries during working, must be provided and coordinated on a national level. These guidelines should
The role of GABAA receptor activity in post-ictal depression period in a rat kindling model of epilepsy
(Brieflands, 2009-03-31) Javad Mirnajafi; Vahid Shibani; MohammadReza PalizVan; Mehdi Sadegh; Tahereh Zeynali
Introduction: Following the seizure there is an inhibitory period named “post-ictal depression period” which has a depressing effect on the following seizure. In this study, the role of GABAA receptors on post-ictal depression period was investigated in amygdala kindling model of epilepsy in rat. Materials and Methods: Animals were kindled by electrical stimulation of amygdala. Then, they were divided into four groups. Different groups of kindled animals were received a second stimulation at 10, 30, 60 and 90 min after the first stimulation and the percentage of suppression of seizure parameters after the second stimulation was calculated. In another four groups, bicucullin, a selective GABAA receptor antagonist (100 nM), was intra-cerebroventricularly microinjected 10 min before the second stimulation and its effect on the percentage of suppression induced by the first stimulation was investigated. Six animals were used in each group. Results: When the second stimulation was applied at 10 and 30 min after the first stimulation a significant reduction was observed in the seizure severity and seizure parameters were depressed after the second stimulation. Bicucullin microinjection significantly decreased the percentage of depression in seizure parameters following the second stimulation compared to the animals received the solvent alone (P
Comparative study of preemptive Ketorolac and Tetracaine eye drops on the need for sedatives and analgesics during and after cataract surgery
(Brieflands, 2022-06-30) Mojgan Mortazavi; Darioush Moradi Farsani; Arian Amirkhosravi; Kamran Montazeri
Introduction: Tetracaine drop is commonly used in ophthalmic surgeries. Ketorolac drop is an anti-inflammatory drug that has been used in some surgical procedures. The present study compared the effect of Ketorolac and Tetracaine eye drops on the need for sedative and analgesic medications during and after cataract surgery. Materials and Methods: In this clinical trial study, 86 patients undergoing cataract surgery were distributed into two equal groups as receiving Tetracaine 0.5% or Ketorolac 0.5% eye drops, one drop every 10 min from 30 min before surgery. Postoperative pain, rescue doses of the sedative drugs, hemodynamic variables, nausea and vomiting, hypoxia, and respiratory depression were evaluated in both groups. Results: Significant differences were not found between the two groups based on hemodynamic variables during surgery and recovery period, postoperative pain up to 24 hours, postoperative nausea and vomiting, and complication such as hypoxia and respiratory distress (P>0.05). Notably, no significant differences were found between the two groups based on midazolam and propofol rescue doses (P>0.05). Conclusion: There were no significant differences between Tetracaine and Ketorolac eye drops regarding the sedation and analgesia requirements and hemodynamic parameters during and after the cataract operation.
Causes of family refusal for organ donation in brain-dead cases, A Narrative Review
(Brieflands, 2023-02-28) Keihan Mostafavi; Mojtaba Mokhber Dezfoli; Negin Jarrah; Sadra Montazeri; Seyed Khashayar Mirbahaeddin; Mahdieh Hazrati; Yazdan Abdolmohammadi; Seyyed Javad Najafizadeh
Introduction: One of the most important barriers to organ transplantation and the consequent mortality of people on the waiting list for transplants in most countries is family refusal. As a result, recognizing the effective factors in family consent will provide a bridge to increase the rate of supply of organs needed for transplantation. Due to the various factors affecting organ donation, including cultural, religious, scientific, social, moral, religious, and legal dimensions, there is a need for comprehensive and up-to-date studies in this field. Therefore, in this narrative review article, we gathered and summarized the major and influential causes of family refusal among the next of kin of brain death cases It is suggested that some ceremonies and social activities be considered to familiarize the community with brain death and the process of organ donation. Also, it is necessary to organize meetings with religious authorities to present the humanitarian aspects of organ donation.
Effects of progressive resistance training on serum levels of vaspin and some inflammatory markers in male rats
(Brieflands, 2012-12-31) Alireza SafarZadeh; Elahe Talebi- Garakani
Introduction: Vaspin (visceral adipose tissue-derived serine protease inhibitor) has been recently identified as a novel adipocytokine with insulin-sensitizing effects. There is a limited information available regarding the effect of exercise training on serum vaspin concentration. The purpose of this study was to investigate the effect of resistance training on serum vaspin levels and some inflammatory markers. Material and Methods: Sixteen male Wister rats (288 ± 19g) were randomly divided into experimental and control groups. The experimental group was subjected to a resistance training program with the use of a ladder (3 days/wk, for 4 wk). Body weight and fasting serum levels of glucose, insulin, vaspin, C-reactive protein (CRP), interleukin (IL)-6, and tumor necrosis factor (TNF)-α were measured. Results: following four weeks resistance training, serum vaspin level was significantly lower in the experimental group than the control group (P< 0.05). The resistance training significantly decreased serum levels of IL-6, CRP and TNF-α in the experimental group as compared to the control group (P< 0.05). Conclusion: This study indicated that resistance training could decrease serum vaspin levels as well as inflammatory markers concentrations. Decreased of serum vaspin levels may be an adjustment mechanism in response to insulin sensitizing improvement and decline of inflammatory markers levels in trained rats.
Pharmacogenomics approach in psychiatry disorders
(Brieflands, 2022-04-30) Negar Sarhangi; Farshad Sharifi; Shekoufeh Nikfar; Mandana Hasanzad
Psychiatric disorders are important health issues in the world, and their management is facing some serious challenges. Drugs that are widely used in the treatment of psychiatric disorders, including antidepressants, antipsychotics, and mood stabilizers, are often associated with many side effects and are adequately effective only in a small proportion of patients. Many factors, including genetic factors, affect the effectiveness of medications prescribed for mental health disorders. Pharmacogenetics is the study of the association between genetic variations in genes encoding carriers, receptors, and drug-metabolizing enzymes with drug response. Many genes have been identified as affecting the drugs responsible for psychiatric disorders and genetic variation can lead to variations in the efficacy and side effects of drugs. This review article summarizes some psychiatric drugs and related genes, which could have potential functional effects.
Assessment the effects of hydroalcoholic extract of Thymus vulgaris on acute pain in hot plate and tail flick in mice
(Brieflands, 2004-06-30) Abbasali Taherian; Ali RashidiPor; Abbas Ali Vafaei; Morteza Jarahi; Hosein MiladiGorji; Mitra Emami; Hossein Sadeghi
Introduction: Since uses of antinoceception chemical drugs for relief of pain have many side effects today medical plants are very noticeable. The present work investigated the effects of Hydoalcoholic extract of Thymus vulgaris )TV( on acute pain in Hot plate and Tail flick models. Material and Methods: Albino mice (20-30 gr) were used for this study. Hydroalcoholic extract of TV seed was injected in doses of 100 and 500 mg/kg 30 min before test. Then the analgesic effect of the drug on acute pain was evaluated using Hot plate and Tail flick models. Results: Results indicated that TV has analgesic effect in both doses in both models (P
Effects of different concentration of Zattariamultiflora essence on immunity system of broilers
(Brieflands, 2016-10-31) Hamid Staji; Khosro Ghazvinian; A. li Mahdavi; Keivan Keramati
Introduction: Zattariamultiflora (Z. multiflora) is a herb belonging to Labiatae family with known therapeutic effects, like anti-inflammatory and antimicrobial activity especially for gastrointestinal tract and respiratory systems and use of such herbs seems to be necessary in today’s medicine. The aim of present study was to evaluate the effects of Z. multiflora essence on immunity of broilers. Materials and Methods: Different concentrations of Z. multiflora were added to the food of broilers, then the antibody titer’s were measured for Sheep Blood Cells (SRBC) and Newcastle Disease virus (NDV) plus stimulation rate of T-lymphocytes by Delayed-Typed Hypersensitivity (DTH) assay Results: Assays revealed that addition of 200 & 400 ppm of Z. multiflora essence in the diet of broilers increased Ab levels against NDV, statistically (p
The study of relationship between the tension-making factors and mental health of Semnan nurses
(Brieflands, 2007-03-31) Imanollah Bigdeli; Saideh KarimZade
Introduction: Nursing is one of the high tensional professions in the health and theraputic services. There are many job stressor, which endanger the mental health of indivduals in this postion. The purpose of this research was to study the relationship between the tension-making factors and mental health among nurses in Semnan. Material and Methods: In the curreny study, 78 of 178 registareted nurses who work at 3 hospitals of Semnan (Amir-al Momenian, Shafa, and Fatemieh hospital), were chosen by radom stratified method. The research was a correlational study. Data was collected using two types of questionnaires general health questionnaire including 28 questions and occupational exhaustion questionnaire including 25 questions. Data analysis were carried out using pearsons coefficient and T-test. Results: The results of present study showed that:1) There was a significant relationship between the job stress and nurses mental health. 2) No significant relationship was observed between male and female nurses with respect to their mental health. 3) There was a significant relationship between married and single nurses with respect to their mental health. 4) A significant relationship was detected between nurses mental health and their occupational history. Conclusion: Given to these findings, nurses encounter to special tensions due to their occupation and their mental health directly influences on quality of functioning in care of patients. Consequently, reducing stressors by psychological and milieu interventions could improve nurses mental health and subsequently their job efficiency.
Orf followed by erythema multiforme
(Brieflands, 2007-12-31) maryam azizzadeh
Orf is a sheep disease due to infcetion with a parapoxvirus. The infection is mainly transmitted to man by direct contacts and seldomly by contaminated instruments. Infection in human results in a benign and self-limited disease, generally. Although, rare complications have been mentioned, this is a report about a case of orf with erythema multiforme in a 54 year old woman.
Morphine withdrawal symptoms improvement by Ziziphora tenuior L. ethanoic extract in male mice
(Brieflands, 2016-09-30) Mojtaba Godarzi; Saeid Abbasi Maleki; Seyed Zahra Mousavi; Nasroallah Moradi Kor
Introduction: Ziziphora tenuior L. (Z. tenuior) has multiple biological activities such as spasmolytic, antidiarrheal, analgesic, antidepressant and sedative effects. In the present study, the effect of ethanolic extract of Z. tenuior on morphine withdrawal symptoms was investigated in the male mice. Materials and Methods: In this study, 40 male mice weighing 20-30 g were used. The animals were divided into five groups of eight. The study groups included negative control or normal saline (10 ml/kg), positive control or clonidine (3.5 mg/kg), and different doses of Z. tenuior (50, 75, and 100 mg/kg). Dependency on morphine was induced by administration of different doses of morphine (50, 50, 75, and 50 mg/kg) in four days. On last day, after administration of single dose of morphine, naloxone (5 mg /kg) was injected and morphine withdrawal signs were observed and recorded within 30 min. All drugs and extracts were injected intraperitoneally with a single volume of 10 ml/kg. Results: Results showed that at all doses (50, 75 and 100 mg/kg) of Z. tenuior extract, significantly decreased the number of jumping (P
Effect of cyproheptadine on combat related posttraumatic stress disorder nightmares
(Brieflands, 2005-03-31) Gholamhossein Ahmadzadeh; GhorbanAli Asadolahi; Javad MahmodiGharaie; Taktom Fhagihi; Nasrollah BasharDost
Introduction: Recent studies have shown the role of serotonergic system in posttraumatic stress disorder (PTSD). Terazodone and nefazodone (5HT2 receptor antagonists) ameliorated PTSD nightmares but the reports are mixed. This study prompted an open trial of cyproheptadine for Iran versus Iraq combat PTSD patient nightmares. Materials and Methods: 25 patients studied in an eight-week, before-after trial of cyproheptadine. The participants were male and chronic PTSD patients with combat related nightmare. The exclusion criteria included current substance abuse or dependence, psychotic disorders and any medical condition that contraindicated the use of cyproheptadine. Results: Five patients were excluded from the study because of side effects including dizziness and somnolence. Twenty patients completed the study. Average of nightmare severity decreased from 6.85 to 5.05, which was statistically significant (P
Effects of increasing walking speed on ankle muscle co-contraction based on spasticity severity in chronic stroke patients
(Brieflands, 2016-10-31) Roghayeh Mohammadi; Solmaz Mohammadzadeh
Introduction: Plantar flexor muscles produce propulsive force in the second half of stance phase deficient motor output from these muscles would lead to inadequate propulsion at push off phase of gait following stroke. The aim of the present study was to examine the effects of walking on a treadmill at varying speeds on ankle muscle activation in stroke survivors and to compare the effect of increasing speed on plantar flexor muscle activity in subject groups in relation to spasticity severity. Materials and Methods: Nineteen stroke survivors (13M/6F) walked on a standard treadmill at three different speeds (self-selected, self-selected+20%, self-selected+40%). The electromyographic activity of Medial Gastrocnemius (MG), and Tibialis anterior (TA) muscles recorded at push off phase of the gait. Results: In the high spasticity subgroup (Tardieu scale ≥2), paretic MG activity increased as walking speed increased (p
Gene cassette design, cloning and expression of recombinant elastin like polypeptide to produce a functional biomaterial in tissue engineering
(Brieflands, 2016-09-30) Mohammad Reza Soleyman; Mostafa khalili; Alireza Soleyman Meigoni; Hadi Mohammadzadeh Ghasghaii; Adib Zendedel; Maryam Baazm
Introduction: Elastin-like polypeptide (ELP) s an artificial biopolymer and a pentapeptide with repeating motif of Val-Pro-Gly-Xaa-Gly (VPGXG). The structure is derived from extracellular cellular matrix (ECM) elastin. Protein-based polymers, which are composed of repeat units of natural or unnatural amino acids, have recently emerged as a promising new class of materials. The unique properties of ELPs such as being genetically encoded in a heterologous host (e.g., bacteria or eukaryotic cell), excitability under the influence of environmental factors, biocompatibility and biodegradability made them popular in a wide variety of biomedical applications. Materials and Methods: In this study, 330bp GRGDS-ELP monomer gene (expressing21 pentapeptide) was oligomerized into the cloning vector pUC to produce 990bp trimer gene (expressing63 fusion pentapeptide).After confirming gene structure by sequencing, the gene sequence was subcloned into pET-MOD expression vector and transduced into the E.coli. Results: Recombinant protein expression was induced by IPTG and the accuracy of the recombinant protein was confirmed by SDS-PAGE and Western blotting. Conclusion: With mentioned method, ELP was obtained for further studies
Expression of ente rotoxigenic Escherichia coli heat-labile toxin B subunit ge ne in Salmonella typhimurium G30: immunization using an oral live delivery system
(Brieflands, 2000-03-30) MohammadReza AkbaryEiedgahi; Bahman Torabi; Veladimir Richinski
Introduction . Attenuated Salmonella strains, such as S. typhimurium G30 have a great potential as live carriers for the de livery of heterologous ant igens to the immune system. Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea among children in developing countries as well as travelers to this area . Heat-l abi le toxin (LT), one of the most important pathogenesis factors of ETEC, is comprised of one A subu nit (LT-A) and five identical B subunit (LT-B). LT-B is responsible for bin ding the toxin to ganglioside GMI-receptor on the surface of int estinal epithelial cells and is a strong immunogen against ETEC mediated diarrhea. The aim of this study was to examine expre ssion of LT-B in S. typhimurium G30 under the control of two various promoter and examina tion of th at as oral live delivery system to elicit antibody resp onse in mice. Materials and Methods. Th e LT-B en coding gene in pTUCLTB and pTRCLTB plasmids were transformed into S. typhimurium G30 and named G30ULTB an d G30TLTB, respectively. Produced level of LT-B by each constructs were measured and structural and immunological characteristics of produced LT-B were studied by GMI ELISA and SDS-PAGE. Finally, ability of these construct s in indu ction of antibody response in C57/BL6 were compared. Results. The results confirmed th at the Salm onell a str ain could produce rLTB with correct conformati on and conserved the main char acteristic including pentameri c form, re ceptor binding ability and interaction with monoclonal antibodies. Our finding also showed an increasing level of antitoxin antibody in mice orally immunized with both G30TLTB and G30ULTB. Conclusion. In conclusion, S. typhimurium G30 could express LT-B in vivo, where the chemical induction is impractical and subsequently induce the antibody response. Modification of this system can result into development of an oral live vaccine against ETEC.
Gene expression data clustering and it’s application in differential analysis of leukemia
(Brieflands, 2008-03-31) Hamid Alavi Majd; Hamid Alavi Majd; Yalda Mehrabi; Bahar Taghavi
Introduction: DNA microarray technique is one of the most important categories in bioinformatics, which allows the possibility of monitoring thousands of expressed genes has been resulted in creating giant data bases of gene expression data, recently. Statistical analysis of such databases included normalization, clustering, classification and etc. Materials and Methods: Golub et al (1999) collected data bases of leukemia based on the method of oligonucleotide. The data is on the internet. In this paper, we analyzed gene expression data. It was clustered by several methods including multi-dimensional scaling, hierarchical and non-hierarchical clustering. Data set included 20 Acute Lymphoblastic Leukemia (ALL) patients and 14 Acute Myeloid Leukemia (AML) patients. The results of tow methods of clustering were compared with regard to real grouping (ALL & AML). R software was used for data analysis. Results: Specificity and sensitivity of divisive hierarchical clustering in diagnosing of ALL patients were 75% and 92%, respectively. Specificity and sensitivity of partitioning around medoids in diagnosing of ALL patients were 90% and 93%, respectively. These results showed a well accomplishment of both methods of clustering. It is considerable that, due to clustering methods results, one of the samples was placed in ALL groups, which was in AML group in clinical test. Conclusion: With regard to concordance of the results with real grouping of data, therefore we can use these methods in the cases where we don't have accurate information of real grouping of data. Moreover, Results of clustering might distinct subgroups of data in such a way that would be necessary for concordance with clinical outcomes, laboratory results and so on.
Evaluation of nutritional status of hemodialysis patients by hand grip strength
(Brieflands, 2022-08-31) Atefeh As’habi; Samin Hejazi; Maryam Mofidinejad; Atena Ramezani; Maryam Nazari
Introduction: Malnutrition (MN) is a typical complication among hemodialysis patients which is associated with morbidity and mortality rates. This cross-sectional study investigated different indices in detecting MN in hemodialysis patients. Materials and Methods: MN assessment was performed using subjective [Subjective Global Assessment (SGA) and Dialysis Malnutrition Score (DMS)] and objective techniques [Hand Grip Strength (HGS)] on patients undergoing hemodialysis 3 times a week in Semnan Province (Iran). SGA and DMS were evaluated by their specific check-lists and HGS analysis was performed using a handgrip dynamometer. The dietary intake of hemodialysis patients was determined using a 4-day dietary recall. Moreover, 4 ml blood sample was obtained from each patient to measure serum biochemical parameters. Results: Amongst 116 enrolled patients, 61 were male and 55 were female. The total prevalence of MN in terms of SGA, DMS, and HGS was 74.2, 58.6, and 63% respectively. Moreover, the association of SGA with age and of DMS with age, gender, and time of hemodialysis were shown. For HGS, not only these factors but also biochemical parameters showed a relationship. This index was associated with serum Albumin and inflammatory marker, hsCRP. Conclusion: Simple and easily accessible tools like handheld dynamometers can verify to be a good nutrition assessment technique for hemodialysis patients in combination with SGA or DMS.
Design and implementation of an intelligent clinical decision support system for diagnosis and prediction of chronic kidney disease
(Brieflands, 2022-08-31) Ali Valinejadi
Introduction: Chronic kidney disease (CKD) is one of the most important public health concerns worldwide. The steady increase in the number of people with End-stage renal disease (ESRD) needing a kidney transplant to survive and incur high costs, highlights early diagnosis and treatment of the disease. This study aimed to design a Clinical Decision Support System (CDSS) for diagnosing CKD and predicting the advanced stage to achieve better management and treatment of the disease. Materials and Methods: In this retrospective and developmental study, we studied the records of 600 suspected CKD cases with 22 variables referred to ShahidLabbafinejad Hospital in Tehran from 2019 to 2020. Data mining algorithms such as Naïve Bayesian, Random Forest, Multilayer Perceptron neural network, and J-48 decision tree were developed based on extracted variables. Then the recital of selected models was compared by some performance indices and 10-fold cross-validation. Finally, the most appropriate prediction model in terms of performance was implemented using the C # programming language. Results: Random Forest classification algorithm with an accuracy of 99.8% and 88.66%, specificity of 100% and 93.8%, the sensitivity of 99.75% and 88.7%, f-measure of 99.8% and 88.7%, kappa score of 99.4% and 82.73%, and ROC of 100% and 90.52% was identified as the best data mining model for CKD diagnosis and prediction respectively. Conclusion: The developed MC-DMK system based random Forestcan be used practically in clinical settings.

Browse

Recent Submissions