Cytotoxic effects of different solvents and essential oil of eucalyptus on human fibroblast cells
| Author | S Samavati | en |
| Author | mahnaz hadizadeh | en |
| Author | M Abedi | en |
| Author | M Kiani Rad | en |
| Issued Date | 2015-12-31 | en |
| Abstract | Background: There are reports that essential oils of different species of eucalyptus have special properties including anti-bacterial, antiviral, anticancer and insecticidal activities. Most of plant essential oils are dissolved in polar or non-polar solvents before examination. Objective: The aim of this study was to determine the cytotoxic effects of different solvents and essential oil of eucalyptus on human fibroblast cells. Methods: This experimental study was performed in the Institute of Biotechnology affiliated to Iranian Research Organization for Science and Technology during 2013-2014. The studied solvents were including ethanol, propylene glycol, dichloromethane, and dimethyl sulfoxide. Fibroblast cells were seeded in 96-well plates. After 24 h incubation at 37ºC, the cells were treated with different concentrations of the solvents or essential oil of eucalyptus (0.05-1 mg/ml). The viability of the cells was determined by the colorimetric MTT assay. Data were analyzed using T-test and One-way ANOVA. Findings: The lowest cytotoxic effect against fibroblast cells was related to 1% ethanol and propylene glycol, while the highest cytotoxic effect was related to 10% dimethyl sulfoxide that decreased cell survival by 47%. Essential oil of eucalyptus also induced fibroblast cell death at concentrations higher than 0.05 mg/ml. Conclusion: For clinical application of essential oil of eucalyptus, using solvent with appropriate concentration is necessary to prevent its cytotoxic effects. | en |
| DOI | https://doi.org/ | en |
| URI | https://brieflands.com/journals/jid/articles/155926 | en |
| Keyword | Eucalyptus | en |
| Keyword | Solvents | en |
| Keyword | Fibroblasts | en |
| Publisher | Brieflands | en |
| Title | Cytotoxic effects of different solvents and essential oil of eucalyptus on human fibroblast cells | en |
| Type | Research Article | en |
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