Prevalence of DNA Mismatch Repair Deficiency in Endometrial Cancer Using Immunohistochemistry

AuthorBehnoush Mehdizadehen
AuthorMasoumeh Ghariben
AuthorAmir Hossein Jafarianen
AuthorMonavvar Afzalaghaeeen
AuthorFateme Homaee Shandizen
AuthorAmirhosein Irajpouren
OrcidBehnoush Mehdizadeh [0000-0003-2501-2684]en
OrcidMasoumeh Gharib [0000-0003-2501-2684]en
OrcidAmir Hossein Jafarian [0000-0002-6488-1519]en
OrcidMonavvar Afzalaghaee [0000-0003-2029-6577]en
OrcidFateme Homaee Shandiz [0000-0002-7718-5387]en
OrcidAmirhosein Irajpour [0000-0001-8265-8268]en
Issued Date2022-08-31en
AbstractBackground: Endometrial cancer (EC) is known as the most common malignancy of the female reproductive system, suggested to be associated with hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch syndrome (LS). Objectives: Therefore, the aim of the present study was to screen for LS in patients with EC using immunohistochemistry (IHC). Methods: In this retrospective cross-sectional study, the patients with EC, referred to Qaem Hospital, Mashhad, Iran, from 2015 - 2019, were enrolled. Paraffin-embedded tissue blocks were then examined via IHC for the expression of four mismatch repair (MMR) proteins, including MLH1, MSH2, MSH6, and PMS2. The demographic and tumor-related data were also extracted from medical records and pathology reports. The data were consequently analyzed at the significance level of P < 0.05. Results: A total number of 100 patients with EC were evaluated using IHC, and 12 (12%) cases were found suspected. As well, no significant relationship was observed between LS and age, tumor site, tumor histology, tumor size, tumor grade, tumor-infiltrating lymphocytes (TILs), and a family/personal history of malignancies. Conclusions: The prevalence of LS based on the IHC expression of the MMR proteins (MLH1, MSH2, MSH6, and PMS2) was 12% in the patients with EC. There was also no significant relationship between the cases suspected and the demographic and tumor-related data.en
DOIhttps://doi.org/10.5812/ijcm-119065en
URIhttps://brieflands.com/journals/ijcm/articles/119065en
KeywordLynch Syndromeen
KeywordEndometrial Canceren
KeywordImmunohistochemistryen
PublisherBrieflandsen
TitlePrevalence of DNA Mismatch Repair Deficiency in Endometrial Cancer Using Immunohistochemistryen
TypeResearch Articleen

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