The Prevalence of Atypical Pneumonia Caused by <i>Mycoplasma pneumoniae</i> (P1 gene) in Patients with Respiratory Infections by Culture and Molecular PCR Methods in Tehran, Iran
| Author | Zahra Babaei | en |
| Author | Iman Pouladi | en |
| Author | Abbass Ashtari | en |
| Author | Gasem Azimi | en |
| Author | Mohammad Niakan | en |
| Orcid | Mohammad Niakan [0000-0003-4239-5015] | en |
| Issued Date | 2019-11-30 | en |
| Abstract | Background: Atypical pneumonia is an upper and lower respiratory tract infection. Mycoplasma pneumoniae is a major cause of community-acquired pneumonia (CAP). Objectives: The present study aimed to determine the prevalence of atypical pneumonia caused by M. pneumonia by culture and molecular PCR methods in Tehran. Methods: In the present study, 102 samples of throat swab were collected from patients with respiratory infections. All samples were cultured in liquid PPLO Broth And solid PPLO agar media (1% glucose and 20% horse serum). The PCR technique with specific primers was implemented after culture and genome extraction through phenol-chloroform technique. Results: In this study, 27 (26.47%) colonies of Mycoplasma were isolated on PPLO agar medium. Using specific primers, it was found that 33 samples (32.4%) were positive in terms of Mycoplasma genus and 14 samples (13.7%) were positive for the presence of M. pneumonia. Conclusions: Mycoplasma pneumonia is a pathogen that causes respiratory tract infections in humans. Molecular PCR method is a quick and sensitive technique that has higher sensitivity and specificity than other methods. The obtained results may contribute to the specific treatment of some patients with symptoms of respiratory infections. | en |
| DOI | https://doi.org/10.5812/jjm.84174 | en |
| Keyword | Atypical Pneumonia | en |
| Keyword | <i>Mycoplasma pneumonia</i> P1 Gene | en |
| Keyword | Culture and Molecular Methods | en |
| Publisher | Brieflands | en |
| Title | The Prevalence of Atypical Pneumonia Caused by <i>Mycoplasma pneumoniae</i> (P1 gene) in Patients with Respiratory Infections by Culture and Molecular PCR Methods in Tehran, Iran | en |
| Type | Research Article | en |