Analysis of Immune Profiles Related to Disease Severity in COVID-19 by Flow Cytometry
| Author | Tugrul Hosbul | en |
| Author | Sema Oren | en |
| Author | Cumhur Artuk | en |
| Author | Canset Nur Aydogan | en |
| Author | Irem Unat | en |
| Author | Serkan Senkal | en |
| Author | Gamze Icen | en |
| Author | Tugba Fatsa | en |
| Author | Ramazan Gumral | en |
| Author | Fatih Sahiner | en |
| Author | Murat Kizilgun | en |
| Author | Mehmet Tevfik Yavuz | en |
| Orcid | Tugrul Hosbul [0000-0002-0150-4417] | en |
| Orcid | Sema Oren [0000-0001-7375-2009] | en |
| Orcid | Cumhur Artuk [0000-0003-0827-990X] | en |
| Orcid | Canset Nur Aydogan [0000-0002-6081-8934] | en |
| Orcid | Irem Unat [0000-0002-9247-2480] | en |
| Orcid | Serkan Senkal [0000-0001-8196-3834] | en |
| Orcid | Gamze Icen [0000-0003-3328-2131] | en |
| Orcid | Tugba Fatsa [0000-0003-3759-495X] | en |
| Orcid | Ramazan Gumral [0000-0002-2303-8234] | en |
| Orcid | Fatih Sahiner [0000-0002-3488-0339] | en |
| Orcid | Murat Kizilgun [0000-0001-5551-4058] | en |
| Orcid | Mehmet Tevfik Yavuz [0000-0002-1964-424X] | en |
| Issued Date | 2023-07-31 | en |
| Abstract | Background: More than 768 million people have been affected by COVID-19. Identifying lymphocyte subsets and cytokine level abnormalities in COVID-19 patients is essential to gain new insights and data on immunity mechanisms against viral infections. Objectives: We used flow cytometry to determine the relationship between disease severity, lymphocyte subsets distribution, and cytokine level alterations in COVID-19 patients. Methods: Totally 94 COVID-19 patients (32 mild, 31 moderate, and 31 severe) and 27 healthy individuals were included in the cross-sectional study. The distribution of peripheral lymphocyte subsets and cytokine levels was assessed by flow cytometry. Results: The percentages of CD56+ natural killer (NK) cells in all patient groups and total T lymphocytes in moderate and severe groups were significantly lower than those in the control group (P < 0.001). Also, IL-2 (P < 0.001), IL-17A (P < 0.001), IL-4 (P < 0.001), IL-6 (P < 0.001), TNF-α (P = 0.004), IP-10 (P < 0.001), IFN-λ1 (IL-29) (P < 0.001), IFN-λ2/3 (IL-28A/B) (P = 0.011), IFN-β (P < 0.001), IL-10 (P < 0.001), and IFN-γ (P < 0.001) levels were statistically higher in patients than in the controls. Conclusions: Our data revealed that increased levels of certain cytokines in peripheral blood contribute to disease severity. Increased CRP (OR: 1.012, %95 CI: 1.002 - 1.023, P = 0.038) and IL-10 (OR: 1.068, %95 CI: 1.000 - 1.141, P = 0.049) levels, decreased CD56+ NK percentage (OR: 0.576, %95 CI: 0.376 - 0.882, P = 0.011) and lymphocyte count (OR: 0.02, %95 CI: 0.001 - 0.368, P = 0.009), and the presence of diabetes mellitus and mechanical ventilation were independent predictors of mortality. | en |
| DOI | https://doi.org/10.5812/jjm-138835 | en |
| Keyword | COVID-19 | en |
| Keyword | Flow Cytometry | en |
| Keyword | Lymphocyte Subsets | en |
| Keyword | Cytokines | en |
| Publisher | Brieflands | en |
| Title | Analysis of Immune Profiles Related to Disease Severity in COVID-19 by Flow Cytometry | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jjm-16-7-138835.pdf
- Size:
- 253.58 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF