Special Real-time PCR and Conventional PCR for Detection of Rhino-Orbital-Cerebral Mucormycosis
| Author | Naghmeh Bahrami | en |
| Author | Hamidreza Jamaati | en |
| Author | Behrooz Farzanegan | en |
| Author | Rezvan Azim Nejad | en |
| Author | Farnoush Mohammadi | en |
| Author | Mraj Naghdi | en |
| Author | Seyedeh Zahra Fotook Kiaei | en |
| Author | Abdolreza Mohamadnia | en |
| Orcid | Abdolreza Mohamadnia [0000-0001-9662-7462] | en |
| Issued Date | 2024-03-31 | en |
| Abstract | Background: Mucormycosis is an invasive fungal infection that occurs in immunodeficiency patients and belongs to infections caused by mucoral fungi, such as Rhizopus and Rhizomucor. Objectives: This study investigated the identification and detection of the mucor fungus in tissue samples and other samples by the real-time polymerase chain reaction (PCR) method. Methods: This was a cross-sectional-descriptive study. A total of 80 tissue samples were collected from referring patients to diagnose fungi with the opinion of a specialist doctor. After extracting deoxyribonucleic acid (DNA) from the samples, PCR and real-time PCR tests were performed using specific primers for mucor and universal-pan and compared to culture. The results were confirmed by sequencing. Results: In this study, 80 samples were examined. In the PCR method, 74 and 75 cases of fungi were confirmed from the DNA obtained from the colonies and tissues, respectively. By using a specific primer for mucor, 12 patients were reported as positive for mucor. Additionally, 76 and 77 cases of fungi were confirmed in the real-time PCR method from the DNA obtained from the colonies and tissues, respectively, and positive cases were reported. By using specific primers and the real-time PCR test for mucor, 16 cases of mucor were reported from colonies, and 15 cases of mucor were reported positive from tissues. Conclusions: This study showed that real-time PCR and PCR are high-speed and more accurate methods than the culture test in identifying its species and have acceptable results in clinical cases. | en |
| DOI | https://doi.org/10.5812/jcma-145366 | en |
| Keyword | Mucor | en |
| Keyword | Detection | en |
| Keyword | PCR | en |
| Keyword | Real-time PCR | en |
| Publisher | Brieflands | en |
| Title | Special Real-time PCR and Conventional PCR for Detection of Rhino-Orbital-Cerebral Mucormycosis | en |
| Type | Research Article | en |
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