Reverse Transcription Loop-Mediated Isothermal Amplification Assay with High Sensitivity to Rapid Detection of Viable <i>Salmonella</i> in Foods
Author | Dong Chen | en |
Author | Fangju Tian | en |
Author | Wanyu Liu | en |
Author | Jingyi Yu | en |
Author | Dafeng Song | en |
Orcid | Dong Chen [0000-0001-8654-9560] | en |
Orcid | Fangju Tian [0000-0002-9070-6861] | en |
Orcid | Wanyu Liu [0000-0001-5555-5503] | en |
Orcid | Jingyi Yu [0000-0002-3255-9250] | en |
Issued Date | 2021-11-30 | en |
Abstract | Background: Salmonella is one of the main foodborne bacterial pathogens, causing diseases and death. The study used reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect Salmonella. Objectives: To design six primers and detect Salmonella using RT-LAMP to facilitate the rapid detection of pathogenic bacteria in food. Methods: We designed six primers based on the gene coding sequences of inv A, specific to Salmonella. Each reaction solution contained 6.0 mM MgSO4, 1 M betaine, 1.6 mM dNTPs, 160 U/mL Bst DNA polymerase, 0.2 μM of both external primers, 0.8 μM of both internal primers, and 0.2 μM of both loop primers. The reaction temperature was 65°C. Results: Our amplified products were separated by 2% agarose gel electrophoresis. The detection limit was 10 CFU per reaction. Conclusions: RT-LAMP exhibited the same accuracy as the Chinese National Standard assay (GB 4789.30-2016) assay in detecting Salmonella in foods. RT-LAMP was highly specific and sensitive; hence, it may serve as an effective tool in detecting Salmonella. | en |
DOI | https://doi.org/10.5812/jjm.117938 | en |
Keyword | <i>Salmonella</i> D012475 | en |
Keyword | RT-LAMP | en |
Keyword | Foodborne Pathogens | en |
Keyword | Rapid Detection | en |
Publisher | Brieflands | en |
Title | Reverse Transcription Loop-Mediated Isothermal Amplification Assay with High Sensitivity to Rapid Detection of Viable <i>Salmonella</i> in Foods | en |
Type | Research Article | en |