lncRNA <i>VLDLR-AS1</i> Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran
| Author | Amin Moqadami | en |
| Author | Alireza Ahmadi | en |
| Author | Mohammad Khalaj-Kondori | en |
| Orcid | Amin Moqadami [0000-0002-5950-599X] | en |
| Orcid | Alireza Ahmadi [0000-0001-7256-0953] | en |
| Orcid | Mohammad Khalaj-Kondori [0000-0001-9231-889X] | en |
| Issued Date | 2023-12-31 | en |
| Abstract | Background: Colorectal cancer (CRC) is the third most common cancer that frequently spreads to other parts of the body, with a low chance of recovery and a high mortality rate. Long non-coding RNAs are considered significant prognostic and diagnostic indicators because they are dysregulated in various cancers and have distinctive expression patterns and high tissue- and cell-specificity. VLDLR-AS1 lncRNA deregulation has been associated with several malignancies. Objectives: This study aimed to evaluate the expression levels of VLDLR-AS1 in CRC. It was the first time this assessment was conducted. Methods: We studied 188 samples, including 94 tumor samples and 94 paired adjacent non-tumor tissues. Total RNA was extracted, and its quantity and purity were assessed. TaKaRa PrimeScript 1st Strand cDNA Synthesis Kit (Kusatsu, Japan) was used for the reaction. The StepOnePlus Real-Time PCR System (Applied Biosystems) was set up to assess the relative expression of VLDLR-AS1. Results: According to the qRT-PCR data, the VLDLR-AS1 expression levels in CRC tissues were significantly lower than in tumor margins (P < 0.0001). In addition, receiver operating characteristic curve analysis demonstrated that VLDLR-AS1 expression can discriminate between tumor and non-tumor samples with the sensitivity and specificity of 72.34% and 51.06%, respectively (P = 0.03, AUC = 0.6274). However, no significant association was found between the expression levels of VLDLR-AS1 and clinicopathological features in CRC patients. Conclusions: The results of this study indicated that VLDLR-AS1 lncRNA is significantly downregulated in the tumor tissues of CRC patients compared to healthy tumor margin tissues. This evidence shows the potential of this gene as a promising biomarker for the early detection of CRC development. | en |
| DOI | https://doi.org/10.5812/jjcmb-141522 | en |
| Keyword | Colorectal Cancer | en |
| Keyword | LncRNA | en |
| Keyword | <i>VLDLR-AS1</i> | en |
| Keyword | Biomarker | en |
| Publisher | Brieflands | en |
| Title | lncRNA <i>VLDLR-AS1</i> Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran | en |
| Type | Research Article | en |
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