IL-23 and IL-27 gene expression in three breast cancer cell lines and metastatic and non-metastatic lymph nodes in breast cancer patients
| Author | Alireza Eskandari | en |
| Author | Mansooreh JabariPor | en |
| Author | Ali Khodadadi | en |
| Author | Abbas Ghaderi | en |
| Orcid | Ali Khodadadi [0000-0003-0442-273X] | en |
| Issued Date | 2011-09-30 | en |
| Abstract | Introduction: There is a great need to identify prognostic and diagnostic cancer bio-markers which can be applied for vaccine and immunotherapy development. The aim of this study was to determine gene expression of IL-23 and IL-27 in two groups: lymph nodes of breast cancer patients and three cell lines. Materials and Methods: IL-23 and IL-27 mRNA transcript were investigated in 10 lymph node samples (consisting of 5 metastasis and 5 non metastasis ones) and three cell lines (including of SKBR3, MDA-MB-468 and MCF7) by quantitative real time PCR (Q-PCR) procedure using designed primers, Master Mix reaction containing SYBER green and β actin housekeeping gene. Results: Data showed no significant differences in IL-23 and IL-27 mRNA gene expressions among metastatic lymph node and non-metastatatic ones. Also, we did not find any significant differences in IL-23 and IL-27 mRNA expressions in cancer cell lines. Conclusions: Our findings indicate that breast cancer microenvironment has no effect on inflammation via either IL-23 gene expression or Il-27. | en |
| DOI | https://doi.org/ | en |
| Keyword | Breast neoplasms, Gene expression, Interleukin-23 27 human, Lymph nodes | en |
| Keyword | نورویشهای پستان، بیان ژنی، اینترلوکین 23، 27 انسانی، گره های لنفاوی | en |
| Publisher | Brieflands | en |
| Title | IL-23 and IL-27 gene expression in three breast cancer cell lines and metastatic and non-metastatic lymph nodes in breast cancer patients | en |
| Type | Research Article | en |
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