Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry
| Author | Fereshteh Mehri | en |
| Author | Firouzeh Nazari | en |
| Author | Zohreh Fasihi | en |
| Author | Farzad Kobarfard | en |
| Issued Date | 2020-10-31 | en |
| Abstract | A sensitive method using ion-pair extraction was developed by liquid chromatography tandem mass spectrometry (LC–MS/MS) for measurement of 4-methylimidazole (4-MI) in NMRI mice plasma and cerebrospinal fluid (CSF). Detection was done by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring (MRM) mode. The validation method was applied to quantification of 4-MI in plasma and CSF samples using oral doses of 100, 200, and 300 mg/kg in NMRI mice. The efficiency of the method was evaluated in terms of linearity (R 2> 0.99), recovery (98–107%, 3 levels) and precision (8–10%, 3 levels, n = 6). Limit of detection (LOD) and limit of quantification (LOQ) were 25 ng/mL and 50 ng/mL, respectively. The results obtained showed that the exposure to oral doses of 4-MI in mice makes different concentrations in plasma and CSF and causes significant changes in mice. This study was the first report for determination of 4-MI in plasma and CSF samples in mice. Our results suggest that LC-MS/MS-based on ion-pair extraction is a robust method with high detection ability for measurement of 4-MI in plasma and CSF samples. Therefore, the developed method can be useful for evaluation and monitoring of imidazole derivatives in biological samples. | en |
| DOI | https://doi.org/10.22037/ijpr.2020.112406.13740 | en |
| Keyword | 4-Methylimidazole | en |
| Keyword | NMRI mice | en |
| Keyword | Plasma | en |
| Keyword | Cerebrospinal fluid | en |
| Keyword | LC-MS/MS | en |
| Publisher | Brieflands | en |
| Title | Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry | en |
| Type | Original Article | en |
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