Prevalence of <i>PVL</i> and <i>LucED</i> Genes in Methicillin-Resistant and Susceptible Isolates of <i>Staphylococcus aureus</i> Isolated from Burn Patients in Kermanshah, Iran

AuthorFeizollah Mansourien
AuthorSiavash Vazirien
AuthorMandana Afsharianen
AuthorMohammad Hossein Zamanianen
AuthorNahid Madadi-Golien
AuthorZainab Mohseni Afsharen
AuthorRonak Miladien
AuthorMohsen Bijarien
AuthorAmirhossain Naghash Zargaren
AuthorFatemeh Nourien
AuthorKamal Ahmadien
AuthorMohsen Azizien
OrcidFeizollah Mansouri [0000-0002-3564-8160]en
OrcidSiavash Vaziri [0000-0002-3907-6155]en
OrcidMandana Afsharian [0000-0002-1075-4805]en
OrcidMohammad Hossein Zamanian [0000-0002-5459-3695]en
OrcidNahid Madadi-Goli [0000-0002-4531-3081]en
OrcidZainab Mohseni Afshar [0000-0002-1085-374X]en
OrcidRonak Miladi [0000-0002-3330-3142]en
OrcidMohsen Bijari [0000-0001-8362-8191]en
OrcidAmirhossain Naghash Zargar [0000-0002-9467-7332]en
OrcidFatemeh Nouri [0000-0002-3879-2539]en
OrcidKamal Ahmadi [0000-0002-9830-6651]en
OrcidMohsen Azizi [0000-0002-3203-5473]en
Issued Date2020-11-30en
AbstractBackground: The main pathogen causing various nosocomial infections, especially in burn patients, is Staphylococcus aureus. An important virulence factor of this bacterium is leukocidin, which binds to white blood cells and leads to apoptosis and necrosis. Objectives: In the present research, we aimed at investigating the prevalence of the panton-valentine leukocidin (PVL) and LucED genes in methicillin-susceptible and resistant isolates of S. aureus isolated from burn patients in Kermanshah, Iran. Methods: Overall, 73 isolates of S. aureus were collected from burn wounds and identified by specific biochemical tests. After determining the susceptibility patterns of the isolates by the disc diffusion method, the frequencies of the PVL and LucED genes were assessed using specific primers and polymerase chain reactions. Results: The rates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA) isolates were 58.9% (43 isolates) and 41.1% (30 isolates), respectively. In MRSA isolates, the highest antibiotic resistance was to penicillin (100%) and gentamicin (81.4%). Moreover, the frequencies of the LucED and PVL genes were 76.7% (56 isolates) and 27.4% (20 isolates), respectively, and the highest frequency of the LucED and PVL genes was observed in MRSA (81.4%) and MSSA (40%) isolates, respectively. Conclusions: Increased multidrug resistance pattern among S. aureus isolates with leukocidin production has led to treatment failure and severe infections. The development of infections can be prevented by the identification of isolates carrying the leukocidin gene, and early detection of MRSA isolates is feasible by using the PVL gene.en
DOIhttps://doi.org/10.5812/jjm.109982en
KeywordBurn Wounden
KeywordLeukocidinen
Keyword<i>Staphylococcus aureus</i>en
PublisherBrieflandsen
TitlePrevalence of <i>PVL</i> and <i>LucED</i> Genes in Methicillin-Resistant and Susceptible Isolates of <i>Staphylococcus aureus</i> Isolated from Burn Patients in Kermanshah, Iranen
TypeResearch Articleen

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