Humoral and Cellular Immunomodulation Induced by Propoxure in C57-bl/6 Mice

Abstract

Propoxure (PPX) is a well-known carbamate insecticide, which has been used for several decades in the world and Iran in agriculture and public health programs. However, there is no clear investigation toward its immunotoxicity as yet. In this study, we examined the effects of subchronic i.p. exposure of PPX on humoral (PFC & HA) and cellular (DTH) responses, and also monitored T-Cell subtypes using FACS technique. Briefly, female C57b1/6 inbred mice were administered PPX (0.2, 2 and 10 mg/kg/day i.p. [5 inj/wk] for 28 days) or positive and negative controls. On the day 28, mice were examined for DTH, PFC and HA responses to SRBC. Splenocyte single cell suspension was used for measuring the spleen CD4/CD8 percentage and absolute number. In vitro lymphocyte proliferation response to non-specific antigen (PHA) was also measured using MTT method.