Non Detection of HIV-1 Proviral DNA in PBMCs of the Neonates Born to Iranian HIV-Infected Mothers in PMTCT Program
| Author | Zahra Habib | en |
| Author | Farah Bokharaei-Salim | en |
| Author | Seyed Jalal Kiani | en |
| Author | Saba Garshasbi | en |
| Author | Saeed Kalantari | en |
| Author | Khadijeh Khanaliha | en |
| Author | Sedigheh Taghinezhad-S | en |
| Author | Seyed Hamidreza Monavari | en |
| Author | Angila Ataei Pirkooh | en |
| Author | Maryam Esghaei | en |
| Orcid | Farah Bokharaei-Salim [0000-0002-5367-0847] | en |
| Orcid | Seyed Jalal Kiani [0000-0002-5499-8924] | en |
| Orcid | Saba Garshasbi [0000-0001-7564-7309] | en |
| Orcid | Khadijeh Khanaliha [0000-0003-3264-8496] | en |
| Orcid | Seyed Hamidreza Monavari [0000-0003-1517-6036] | en |
| Orcid | Maryam Esghaei [0000-0003-2944-2147] | en |
| Issued Date | 2021-04-30 | en |
| Abstract | Background: Early diagnosis of immunodeficiency virus-1 infection in children and access to treatment for this infection is critical in decreasing infant mortality. Objectives: The aim of the current survey was to determine the presence of HIV-1 genomic RNA in plasma and proviral DNA in peripheral blood mononuclear cell (PBMC) specimens of neonates born to HIV-infected mothers. Methods: From March 2014 to February 2018, 73 neonates born to HIV-1-infected mothers covered by the prevention of mother-to-child transmission (PMTCT) program were enrolled in this study to compare two different diagnostic methods. After the extraction of viral RNA of plasma and genomic DNA of PBMC specimens, HIV-1 RNA and proviral DNA was tested by amplification of the long terminal repeat (LTR) region of HIV-1 using real-time PCR. Results: Out of 73 evaluated infants, 41 infants (56.2%) were male. The average age of the mothers with HIV-1 infection was 30.7 ± 5.2 (range: 19–47) years. The results revealed that none of the infants were infected with HIV-1, and also all were negative for HIV-1 genomic RNA in plasma specimen and proviral DNA of HIV-1 in PMBC samples. During the present study, 20 infants born to HIV-1 positive mothers who were not included in the PMTCT project were accidentally identified. Four infants (20%) out of these 20 infants were infected with HIV, all were infected with CRF35-AD of HIV, and none carried variants with surveillance drug-resistant mutations. Conclusions: The results of the present study showed that two molecular methods of detecting HIV infection (presence of genomic RNA of HIV-1 in plasma and proviral DNA of HIV-1 in PBMC specimens) are completely in agreement with each other, and the PMTCT program is possibly an effective program. | en |
| DOI | https://doi.org/10.5812/pedinfect.105098 | en |
| Keyword | Human Immunodeficiency Virus Type 1 (HIV-1) | en |
| Keyword | Prevention of Mother-to-Child Transmission (PMTCT) | en |
| Keyword | Peripheral Blood Mononuclear Cells (PBMCs) | en |
| Keyword | HIV-1 Proviral DNA | en |
| Keyword | Neonates | en |
| Keyword | Surveillance Drug-Resistant Mutations (SDRMs) | en |
| Publisher | Brieflands | en |
| Title | Non Detection of HIV-1 Proviral DNA in PBMCs of the Neonates Born to Iranian HIV-Infected Mothers in PMTCT Program | en |
| Type | Research Article | en |
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