Molecular Assay of <i>Staphylococcal</i> Enterotoxin E in Synovial Fluid of Patients with Rheumatoid Arthritis
| Author | Mahdi Rashki | en |
| Author | Ramezan Ali Ataee | en |
| Author | Gholam Hossien Alishiri | en |
| Author | Davoode Esmaeili | en |
| Orcid | Ramezan Ali Ataee [0000-0001-7125-6140] | en |
| Issued Date | 2018-01-31 | en |
| Abstract | Background: The role of Staphylococcal enterotoxin E (Superantigen E) in rheumatoid arthritis pathogenesis has been considered. This paper aimed at determining Staphylococcal enterotoxin E in the synovial fluid of rheumatoid arthritis patients. Methods: In this study, 100 synovial fluid samples of patients with rheumatoid arthritis were examined. The primers pairs were designed based on the S. baureus enterotoxin type E (entE) gene, GenBank: M21319.1. All samples were subjected to DNA extraction separately. Then, polymerase chain reaction (PCR) was implemented. Data were analyzed by descriptive statistics. Results: The PCR results indicated that Staphylococcal enterotoxin E gene existed in synovial fluid samples of 25% of patients with rheumatoid arthritis, with a high percentage. Conclusions: The study results revealed that a high percentage of patients with rheumatoid arthritis have Staphylococcal enterotoxin type E gene in their synovial fluid. However, further studies are needed to assess other Staphylococcal enterotoxins. This finding may provide a model for diagnosing rheumatoid arthritis disease. The results of this study have presented some evidence regarding endogenous origin of involved superantigen in patients with rheumatoid arthritis. | en |
| DOI | https://doi.org/10.5812/iji.14230 | en |
| Keyword | Enterotoxin E | en |
| Keyword | Rheumatoid Arthritis | en |
| Keyword | PCR | en |
| Keyword | <i>Staphylococcus aureus</i> | en |
| Publisher | Brieflands | en |
| Title | Molecular Assay of <i>Staphylococcal</i> Enterotoxin E in Synovial Fluid of Patients with Rheumatoid Arthritis | en |
| Type | Research Article | en |