Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 <i>Pseudomonas aeruginosa</i> at Ardabil Hospitals
| Author | Somayeh Safarirad | en |
| Author | Mohsen Arzanlou | en |
| Author | Jafar Mohammadshahi | en |
| Author | Hamid Vaez | en |
| Author | Amirhossein Sahebkar | en |
| Author | Farzad Khademi | en |
| Orcid | Mohsen Arzanlou [0000-0002-4233-9497] | en |
| Orcid | Jafar Mohammadshahi [0000-0001-9862-4118] | en |
| Orcid | Hamid Vaez [0000-0002-0690-0654] | en |
| Orcid | Amirhossein Sahebkar [0000-0002-8656-1444] | en |
| Orcid | Farzad Khademi [0000-0001-6181-4903] | en |
| Issued Date | 2021-03-31 | en |
| Abstract | Background: Carbapenems are the most commonly administered drugs for the treatment of multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) infections. However, carbapenem-resistant P. aeruginosa is spreading rapidly and has led to a new threat to human health worldwide. Objectives: The current study aimed to determine the prevalence of imipenem-resistant P. aeruginosa, detect metallo-β-lactamase (MBL)-producer isolates, and evaluate their clonal relationships in strains isolated from patients referring to the hospitals of Ardabil city, Iran. Methods: The resistance rate to imipenem was evaluated using the disk diffusion method. Double-disk synergy test and PCR technique were used for phenotypic and genotypic screening of MBL-positive P. aeruginosa, respectively. Ultimately, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and multilocus sequence typing (MLST) methods were used for assessing clonal relatedness among the isolates. Results: The prevalence of imipenem-resistant P. aeruginosa strains was estimated at 57.1% (48 out of 84 isolates). In addition, 45 (93.7%) out of 48 imipenem-resistant P. aeruginosa isolates were phenotypically screened as MBL-positive, among which 16 (35.5%) and three (6.6%) isolates harbored blaIMP and blaVIM-1 genes, respectively. However, blaNDM, blaSIM-2, blaSPM, and blaGIM-1 genes were not detected in this study. MBL-producing P. aeruginosa strains were divided into 42 ERIC-PCR types. Based on the results of MLST, P. aeruginosa ST235 was the only identified sequence type. Conclusions: Our results revealed a high and alarming prevalence of imipenem-resistant and blaIMP-positive P. aeruginosa ST235 at Ardabil hospitals. Continuous monitoring is essential to control the further spread of this highly virulent and drug-resistant clone. | en |
| DOI | https://doi.org/10.5812/jjm.115819 | en |
| Keyword | <i>Pseudomonas aeruginosa</i> | en |
| Keyword | Imipenem | en |
| Keyword | Drug Resistance | en |
| Keyword | Metallo-β-Lactamase | en |
| Keyword | Clonal Relation | en |
| Publisher | Brieflands | en |
| Title | Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 <i>Pseudomonas aeruginosa</i> at Ardabil Hospitals | en |
| Type | Research Article | en |