Hydroalcoholic Extract of Fumaria Parviflora L. Can Potentially Detoxificate HepG2 Cell Line Following Carbon Tetrachloride Exposure
| Author | Amir Abdolmaleki | en |
| Author | Mohsen Zhaleh | en |
| Author | Ali Ghanbari | en |
| Author | Iraj Rashidi | en |
| Author | Cyrus Jalili | en |
| Author | Fateme Feizi | en |
| Author | Fuzieh Khani Hematabadi | en |
| Orcid | Ali Ghanbari [0000-0002-8080-2809] | en |
| Orcid | Cyrus Jalili [0000-0002-5097-7974] | en |
| Orcid | Fuzieh Khani Hematabadi [0000-0002-3860-5713] | en |
| Issued Date | 2024-12-31 | en |
| Abstract | Background: Fumaria Parviflora L. (FP) is a well-known herb in Iranian traditional medicine, widely used for gastrointestinal diseases. Additionally, the detrimental effects of carbon tetrachloride (CT) on the liver and kidneys have been scientifically established. Objectives: This study was designed to assess the therapeutic effects of FP on HepG2 cells exposed to CT toxicity. Methods: After treatment with hydroalcoholic extract of the FP plant and/or CT, cell viability (using the MTT technique and lactate dehydrogenase assay) and apoptosis (using the diphenylamine assay and Annexin V/PI staining) were investigated. The ratio of BCL2/BAX mRNA expression levels was measured using real-time PCR. Data were analyzed using SPSS software. Results: A significant (P < 0.05) reduction in cell viability was observed at doses of 100 and 200 µg/mL of FP in HepG2 cells. The decreased survival rate following CT exposure was concentration-dependent and significant (P < 0.05). The IC50 of CT was determined to be 1.05 μg/mL for 24 hours. Effective detoxification associated with CT was found at 6.25 µg/mL in both the pre-treatment and simultaneous treatment groups. Cell apoptosis was significantly (P < 0.05) decreased following FP administration in CT-treated cells, with an increase in the ratio of BCL2/BAX mRNA expression levels. Conclusions: The hydroalcoholic extract of FP demonstrated hepatoprotective effects on CT-treated HepG2 cells. | en |
| DOI | https://doi.org/10.5812/jcrps-149140 | en |
| URI | https://brieflands.com/journals/jcrps/articles/149140 | en |
| Keyword | Carbon Tetrachloride | en |
| Keyword | Hepatotoxicity | en |
| Keyword | Traditional Medicine | en |
| Keyword | <i>Fumaria Parviflora</i> L. | en |
| Publisher | Brieflands | en |
| Title | Hydroalcoholic Extract of <i>Fumaria Parviflora</i> L. Can Potentially Detoxificate HepG2 Cell Line Following Carbon Tetrachloride Exposure | en |
| Type | Research Article | en |
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