Effects of Different Environmental Factors and Virulence Factors, <i>dupA</i> and <i>iceA</i> Genes, of <i>Helicobacter pylori</i> on Peptic Ulcer

Abstract

Background: Helicobacter pylori is colonized in stomach and has a significant etiological role in different kinds of digestive diseases. Helicobacter pylori has the ability to devastate duodenal mucus causing peptic ulcer. Breakage of stomach lining is also seen more often among people who accustomed to consume tobacco, spicy (or sour) foods, alcohol, coffee, and tranquilizers as well as those who are exposed to heavy meals and stressful-life events (stress ulcer). Also, it seems dup A and ice A genes are involved in H. pylori etiology. Objectives: This study aimed to determine the relation of jhp0917 and jhp0918 genes, ABO blood type, and some environmental factors with peptic ulcer. Methods: In our study, 500 gastric mucosal biopsy samples from digestive tract were examined by rapid urease test (RUT) and 16s rDNA PCR test for H. pylori infection followed by histopathological examinations. The dup A and ice A genes were also evaluated by PCR. Results: In H. pylori colonized peptic ulcers, dup A gene showed no significant relation with peptic ulcer. On the other hand, iceA 1 (+)/ iceA 2 (-) genotype of H. pylori showed a strong association with peptic ulcer (P < 0.05). Conclusions: ABO blood group showed no association with H. pylori infection. Peptic ulcer had a significant relationship with consumption of tobacco (P < 0.05), consumption of tranquilizers (P < 0.05), and meteorism (P < 0.05).