Synthesis and Cytotoxic Evaluation of 6-Amino-4-Aryl-3-Methyl- 2,4-Dihydropyrano[2,3-C]Pyrazole-Carbonitrile Derivatives Using Borax with Potential Anticancer Effects

AuthorHadi Adibien
AuthorLeila Hosseinzadehen
AuthorSepideh Farhadien
AuthorFarahnaz Ahmadien
Issued Date2013-12-31en
AbstractA green and efficient one-pot, four-component synthesis of 6-amino-4-aryl-3-methyl- 2,4-dihydropyrano[2,3-c]pyrazole-carbonitrile derivatives catalyzed by borax in water has been examined and described. This method has several advantages such as environmental friendliness, shorter reaction time, excellent yields, and simple workup procedure. The in vitro cytotoxic activity of the synthesized compounds was investigated against cancer cell lines (SW48, A549, KB, HepG2) in comparison with doxorubicin, a well-known anticancer drug, using MTT colorimetric assay. The synthesized compounds showed good and reasonable cytotoxicity compared with doxorubicin in some studied cell lines. The compounds 5b, 5c, 5g in KB cell line (IC50 = 8±2.217 μM, 7±2.77 μM, 7.5±1.49 μM respectively), 5f in A549 cell line (IC50 = 31.5±2.02 μM), 5g in HepG2 cell line (IC50 = 22.5±3.09 μM), 5e, and 5i in SW48 cell line (IC50 = 23±0.772 μM, 23±4.97 μM respectively) showed the best results in close to the control drug (IC50 = 6.8±0.78 μM, 6.3±0.65 μM, 5.4±0.5 μM, 4.3±0.12 μM in A549, HepG2, KB, and SW48 cell lines respectively). en
DOIhttps://doi.org/en
Keywordsynthesisen
Keywordcytotoxic activityen
KeywordPyranopyrazoleen
KeywordBoraxen
KeywordMTT colorimetric assayen
PublisherBrieflandsen
TitleSynthesis and Cytotoxic Evaluation of 6-Amino-4-Aryl-3-Methyl- 2,4-Dihydropyrano[2,3-C]Pyrazole-Carbonitrile Derivatives Using Borax with Potential Anticancer Effectsen
TypeResearch Articleen

Files