Evaluate the Modified Carbapenem Inactivation Method for Phenotypic Detection of Carbapenemase Production Among Gram-Negative Bacteria
| Author | Shahnaz Armin | en |
| Author | Abdollah Karimi | en |
| Author | Masoud Alebouyeh | en |
| Author | Sedigheh Rafiei Tabatabaei | en |
| Author | Maryam Rajabnejad | en |
| Author | Roxana Mansour Ghanaiee | en |
| Author | Seyed Alireza Fahimzad | en |
| Author | Nafiseh Abdollahi | en |
| Author | Leila Azimi | en |
| Orcid | Shahnaz Armin [0000-0002-4993-482X] | en |
| Orcid | Abdollah Karimi [0000-0002-4225-0097] | en |
| Orcid | Masoud Alebouyeh [0000-0001-7474-2515] | en |
| Orcid | Sedigheh Rafiei Tabatabaei [0000-0003-1723-9424] | en |
| Orcid | Roxana Mansour Ghanaiee [0000-0001-7475-0240] | en |
| Orcid | Seyed Alireza Fahimzad [0000-0001-6054-0656] | en |
| Orcid | Nafiseh Abdollahi [0000-0002-1820-4680] | en |
| Orcid | Leila Azimi [0000-0002-7216-2530] | en |
| Issued Date | 2022-10-31 | en |
| Abstract | Background: Global growing infections by multi-drug resistance (MDR) or extensively drug-resistant (XDR) bacteria are a serious public health problem which can increase the rate of mortality and morbidity even in children. Carbapenem is the last choice therapy in case of antibiotic-resistant bacteria presence. Objectives: This study aimed to evaluate the easy to use method to identify carbapenemase producing bacteria which include in CLSI. Methods: In this descriptive study, 125 carbapenem-resistant and 97 carbapenem-susceptible gram-negative bacteria were included. PCR was used to identify carbapenemase enzymes include VIM, IMP, KPC, NDM-1, SPM-1, OXA-48 as a gold standard method. The modified carbapenem inactivation method (mCIM) was employed to phenotypically identify carbapenemase-producing bacteria. Some modifications were made to the CLSI proposed mCIM to ensure more accurate results in contrast of PCR. Results: The OXA-48 is the most prevalent detected carbapenemase and SPM-1 was not detected in any of strain. The results of the mCIM according to CLSI guide line demonstrated 100% sensitivity to define carbapenemase-producing bacteria. However, in the cases of non-carbapenemase-producing bacteria, only 4% of mCIM test results were consistent with the outcome of PCR. Decrease of the incubation time and the consider 15mm as a break point could increase the accuracy of mCIM against PCR. Conclusions: The results of this study endorse that mCIM test is a valuable method to detect carbapenemase producing bacteria if the three hours consider instead of 4 hours with 15mm break point. | en |
| DOI | https://doi.org/10.5812/pedinfect-121814 | en |
| Keyword | Modified Carbapenem Inactivation Method | en |
| Keyword | Carbapenemase Producing | en |
| Keyword | Gram-Negative Bacteria | en |
| Publisher | Brieflands | en |
| Title | Evaluate the Modified Carbapenem Inactivation Method for Phenotypic Detection of Carbapenemase Production Among Gram-Negative Bacteria | en |
| Type | Research Article | en |