Optimization of Culture Conditions for Enrichment of <i>Saccharomyces cerevisiae</i> with Dl-α-Tocopherol by Response Surface Methodology
| Author | Morteza Mohajeri Amiri | en |
| Author | Mohammad Reza Fazeli | en |
| Author | Mohsen Amini | en |
| Author | Nasim Hayati Roodbari | en |
| Author | Nasrin Samadi | en |
| Issued Date | 2017-10-31 | en |
| Abstract | Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract, and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with dl-α-tocopherol was produced as an accumulator and transporter of a lipid soluble vitamin for the first time. By using one variable at the time screening studies, three independent variables were selected. Optimization of the level of dl-α-tocopherol entrapment in S. cerevisiae cells was performed by using Box-Behnken design via design expert software. A modified quadratic polynomial model appropriately fit the data. The convex shape of three-dimensional plots reveal that we could calculate the optimal point of the response in the range of parameters. The optimum points of independent parameters to maximize the response were dl-α-tocopherol initial concentration of 7625.82 µg/mL, sucrose concentration of 6.86 % w/v, and shaking speed of 137.70 rpm. Under these conditions, the maximum level of dl-α-tocopherol in dry cell weight of S. cerevisiae was 5.74 µg/g. The resemblance between the R-squared and adjusted R-squared and acceptable value of C.V% revealed acceptability and accuracy of the model. | en |
| DOI | https://doi.org/10.22037/ijpr.2017.2146 | en |
| URI | https://brieflands.com/journals/ijpr/articles/125060 | en |
| Keyword | Optimization | en |
| Keyword | Enrichment | en |
| Keyword | <i>Saccharomyces cerevisiae</i> | en |
| Keyword | dl-α-tocopherol | en |
| Keyword | Response Surface Methodology | en |
| Keyword | Box-Behnken | en |
| Publisher | Brieflands | en |
| Title | Optimization of Culture Conditions for Enrichment of <i>Saccharomyces cerevisiae</i> with Dl-α-Tocopherol by Response Surface Methodology | en |
| Type | Original Article | en |
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