Immobilization of Glucose oxidase on nano structure of graphene applied in blood glucose sensors

Abstract

Background: Enzymes Immobilization methods on the fixed bed are very important for recovery, reuse and also increasing catalytic activity of enzyme. In the beginning of this review we define immobilization, then methods of enzyme immobilization are presented. Then immobilization of glucose Oxidase (Gox) on grapheme substrate and determination of glucose by this catalyst is described. Gox is a biocatalyst that accelerates the reaction of Hydrogen Peroxide production from glucose. Most of the conventional glucometers for sensing blood glucose follow this method. In this review, all the recent articles correspond to new advantages of glucose sensing based on graphene substrate are discussed.