Pulsed Magnetic Fields Cause hes1 and hsa-Circ-0068530 Expression Changes in Gastric Cancer Cell Line and Human Normal Fibroblast Cell Line
| Author | Fereshteh Mansoury | en |
| Author | Soheila Abdi | en |
| Author | Nahid Babaei | en |
| Author | Maliheh Entezari | en |
| Author | Abbas Doosti | en |
| Author | Fatemeh Mashayekhi | en |
| Orcid | Soheila Abdi [0000-0001-5525-6378] | en |
| Orcid | Maliheh Entezari [0000-0003-4191-3351] | en |
| Orcid | Abbas Doosti [0000-0001-8052-5963] | en |
| Issued Date | 2021-09-30 | en |
| Abstract | Background: In recent years, the relationship between cancer cells and electromagnetic radiation has received much attention. Objectives: The present study aimed to evaluate the effects of different intensities of electromagnetic fields on gastric cancer cell lines (AGS). Methods: After preparing AGS and Hu02 (normal) cell lines, they were exposed to magnetic flux densities of 0.25, 0.5, 1, and 2 millitesla (mT) for 18 h. The cell viability was studied by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression levels of hes1 and hsa-circ-0068530 RNAs were studied by the quantitative Real-time-PCR technique. Results: The inhibition of gastric cancer cell line growth was observed under the influence of electromagnetic fields at different intensities. However, they did not affect the viability of normal cells. A sharp increase in the expression of hes1 and hsa-circ-0068530 genes was observed in normal cells exposed to 2 mT electromagnetic fields. Conclusions: In general, it can be concluded that the effect of electromagnetic fields on gastric cancer cells depends on their intensity. Magnetic flux densities of 0.25 and 0.5 mT had anti-cancer effects and magnetic flux density of 2 mT showed carcinogenic effects. | en |
| DOI | https://doi.org/10.5812/jjcmb.118967 | en |
| URI | https://brieflands.com/journals/jjcmb/articles/118967 | en |
| Keyword | Gastric Cell Line (AGS) | en |
| Keyword | Magnetic Field | en |
| Keyword | hes1 Expression | en |
| Keyword | Hsa-circ-0068530 Expression | en |
| Publisher | Brieflands | en |
| Title | Pulsed Magnetic Fields Cause hes1 and hsa-Circ-0068530 Expression Changes in Gastric Cancer Cell Line and Human Normal Fibroblast Cell Line | en |
| Type | Research Article | en |