The Effect of Lipopolysaccharide-Stimulated Adipose-Derived Mesenchymal Stem Cells on NAFLD Treatment in High-Fat Diet-Fed Rats
| Author | Reza Afarin | en |
| Author | Fereshteh Aslani | en |
| Author | Shahla Asadizade | en |
| Author | Bahar Jaberian Asl | en |
| Author | Mehrnoosh Mohammadi Gahrooie | en |
| Author | Elham Shakerian | en |
| Author | Akram Ahangarpour | en |
| Orcid | Reza Afarin [0000-0003-3644-0668] | en |
| Orcid | Elham Shakerian [0000-0001-5437-0056] | en |
| Issued Date | 2023-12-31 | en |
| Abstract | Background: Nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) are 2 common liver diseases that currently lack effective treatment options. Objectives: This study aimed to investigate the effect of lipopolysaccharide (LPS)-stimulated adipose-derived stem cells (ADSCs) on NAFLD treatment in an animal model. Methods: Male Wistar rats were fed a high-fat diet (HFD) to induce NAFLD for 7 weeks. The rats were then categorized into 3 groups: Mesenchymal stem cell (MSC), MSC + LPS, and fenofibrate (FENO) groups. Liver and body weight were measured, and the expression of genes involved in fatty acid biosynthesis, β-oxidation, and inflammatory responses was assessed. Results: Lipopolysaccharide-stimulated ADSCs were more effective in regulating liver and body weight gain and reducing liver triglyceride (TG) levels compared to the other groups. Treatment with LPS-stimulated ADSCs effectively corrected liver enzymes, including alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and lipid factors, including low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) values, better than treatment with both FENO and MSCs. ADSCs + LPS treatment significantly decreased transforming growth factor β (TGF-β) and genes associated with inflammatory responses. Additionally, there was a significant reduction in reactive oxygen species (ROS) levels in the rats treated with ADSCs + LPS. Conclusions: Lipopolysaccharide-stimulated ADSCs showed potential in alleviating NAFLD by reducing inflammatory genes and ROS levels in HFD rats, demonstrating better results than treatment with ADSCs and FENO groups alone. | en |
| DOI | https://doi.org/10.5812/ijpr-134807 | en |
| URI | https://brieflands.com/journals/ijpr/articles/134807 | en |
| Keyword | NAFLD/NASH | en |
| Keyword | TGF-β | en |
| Keyword | ADSCs | en |
| Keyword | Lipopolysaccharide | en |
| Keyword | Inflammation | en |
| Publisher | Brieflands | en |
| Title | The Effect of Lipopolysaccharide-Stimulated Adipose-Derived Mesenchymal Stem Cells on NAFLD Treatment in High-Fat Diet-Fed Rats | en |
| Type | Research Article | en |