Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate <i>Salicola marasensis </i>
| Author | Nika Khoshnevis | en |
| Author | Shahla Rezaei | en |
| Author | Amene Samaei-Nouroozi | en |
| Author | Mohsen Amin | en |
| Author | Mahsa Moshfegh | en |
| Author | Mohammad Reza Khoshayand | en |
| Author | Mohammad Ali Faramarzi | en |
| Issued Date | 2018-10-31 | en |
| Abstract | Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was followed with the aim of improving the enzyme production yield. Fractional factorial and Box-Behnken designs were applied to get a 3.4 fold (from 6.0 to 20.3 U mL−1) improvement of protease production. The distinguishing features of this enzyme were stability at a wide range of pH (5.0–11.0) and temperature (25–60 °C), significant compatibility towards organic solvents, metal ions, chemicals, and surfactants, and hydrolysis of a variety of substrates. The properties of this enzyme can be of tremendous help in terms of the halophilic proteolytic extract’s industrial applications. | en |
| DOI | https://doi.org/10.22037/ijpr.2018.2311 | en |
| Keyword | Salicola marasensis | en |
| Keyword | Halophile | en |
| Keyword | Proteolytic extract | en |
| Keyword | Protease activity | en |
| Keyword | Optimization | en |
| Keyword | Characterization | en |
| Publisher | Brieflands | en |
| Title | Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate <i>Salicola marasensis </i> | en |
| Type | Original Article | en |