Genomic Characterization of Multidrug-Resistant Carbapenemase-Producing <i>Enterobacter cloacae</i> ECL189, Co-producing KPC-2, NDM-1, TEM-1, TEM-95, and SHV-66
| Author | Na Du | en |
| Author | Shumin Liu | en |
| Author | Jing Yao | en |
| Author | Kai Yang | en |
| Author | Yun Lin | en |
| Author | Min Niu | en |
| Author | Qingwen Zhang | en |
| Author | Zhiping Yin | en |
| Author | Huanqin Li | en |
| Author | Yan Du | en |
| Orcid | Jing Yao [0000-0002-4571-5486] | en |
| Orcid | Yan Du [0000-0003-2962-6907] | en |
| Issued Date | 2020-11-30 | en |
| Abstract | Background: Carbapenem-resistant Enterobacteriaceae (CRE) has become a public health threat due to resistance to multiple antibiotics. The production of β-lactamase is the most important resistance mechanism of Enterobacteriaceae. Although isolates producing KPC-2 or NDM-1 enzymes have been reported widely, isolates co-producing KPC-2, NDM-1, TEM-1, TEM-95, SHV-66, and other β-lactamases have rarely been detected in the same strain, especially in Enterobacter cloacae. Objectives: In this study, we identified and sequenced the genome of carbapenem-resistant E. cloacae ECL189 to in-depth analyze the resistance and transmission mechanisms of E. cloacae. Methods: We investigated the antimicrobial susceptibility of ECL189 by a VITEK 2 system, E-test gradient strips, and K-B method. Whole-genome sequencing was used by the PacBio RS II platform and Illumina HiSeq 4000 platform. Antimicrobial resistance genes, virulence genes, non-coding RNA, and repeat sequences were predicted by biological information databases. A PCR was used to further confirm that the blaKPC-2, blaNDM-1, blaTEM-1, blaTEM-95, and blaSHV-66 genes existed in ECL189. A conjugation experiment was performed to determine the transferability of resistance. Molecular typing of ECL189 was done by multilocus sequence typing (MLST). Results: Enterobacter cloacae ECL189 was resistant to 21 out of 23 tested antibiotics, but its transconjugant was resistant to 10 out of 18 tested antibiotics. The genome of ECL189 consisted of a 5,026,406 bp chromosome and four circular plasmids. In total, 26 resistance genes and 58 resistance proteins were identified. In addition, 77 determinants associated with bacterial virulence were identified. A large number of resistance and virulence genes were located in the plasmids. The results of whole-genome sequencing were consistent with the β-lactamase genes. The MLST analysis revealed that this strain belonged to ST74. Conclusions: This study further revealed the resistance, virulence, and transmission mechanisms of carbapenem-resistant E. cloacae. Resistance and virulence genes spread in bacteria by the horizontal transfer of plasmids, which should attract more attention in relevant departments. | en |
| DOI | https://doi.org/10.5812/jjm.105761 | en |
| Keyword | <i>Enterobacter cloacae</i> | en |
| Keyword | Whole-Genome Sequencing | en |
| Keyword | BlaKPC-2 | en |
| Keyword | BlaNDM-1 | en |
| Keyword | Carbapenems | en |
| Publisher | Brieflands | en |
| Title | Genomic Characterization of Multidrug-Resistant Carbapenemase-Producing <i>Enterobacter cloacae</i> ECL189, Co-producing KPC-2, NDM-1, TEM-1, TEM-95, and SHV-66 | en |
| Type | Research Article | en |