Survey of Sensitivity of Wet smear and Dorset medium in comparison with Diamond medium for Diagnosis of Trichomonas vaginalis

Abstract

In most references, diamond medium is introduced as one of the best diagnostic methods for Trichomonas vaginalis (Tv), however preparation of this medium is complicated and expensive. A total of 597 vaginal specimens from women, referred to gynecology clinic in Quds maternity hospital of Zahedan, were examined by ongoing three methods including, Wet smear, Dorset medium, Diamond medium. Twenty-eight cases (4.5%), 33 cases (5.3%), 36 cases (5.7%) out of 597 samples were detected by Wet smear, Dorset and Diamond culture media, respectively. The sensitivity of Wet smear and Dorset medium against Diamond medium was 75% and 83.3%, respectively. McNemar test showed no statistical difference between Dorset and diamond culture. It was concluded that the Dorset medium is preferable to the other methods studied here. Considering the facilities of the clinical centers and laboratories, this method is recommended to be used routinely for the diagnosis of T.v in clinical samples.