Diagnostic Accuracy of Enzyme-Linked Immunosorbent Assay Compared with Polymerase Chain Reaction in Diagnosing <i>Helicobacter pylori</i> in Patients with Gastric Problems
| Author | Forouzan Rostami | en |
| Author | Golshid Javdani Shahedin | en |
| Author | Haniyeh Bashizadeh-Fakhar | en |
| Author | Samaneh Divsalar | en |
| Author | Melika Jalalian | en |
| Orcid | Forouzan Rostami [0000-0002-6729-3202] | en |
| Orcid | Haniyeh Bashizadeh-Fakhar [0000-0002-0396-461X] | en |
| Orcid | Melika Jalalian [0000-0001-5001-0918] | en |
| Issued Date | 2024-12-31 | en |
| Abstract | Background: Helicobacter pylori, identified in 1982, is a significant gastric pathogen affecting a large portion of the global population. Objectives: This study aims to compare the diagnostic accuracy of enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) methods for detecting H. pylori, which is crucial for improving treatment strategies for gastric disorders. Methods: The study involved 70 patients at Khurshid Private Laboratory in Tehran between July and September 2022. The goal was to identify H. pylori DNA in stool samples using real-time PCR, while simultaneously testing serum samples for H. pylori using the ELISA method. Data were analyzed using SPSS software. Results: In a study of 70 patients with stomach diseases, the prevalence of H. pylori infection based on PCR results was 24.29%, with a slightly higher prevalence in females than males. For diagnosing H. pylori infection, the optimal IgA cut-off point was identified as 7.15 mg/dL, yielding a sensitivity of 88%, specificity of 89%, and an AUC of 0.94. The optimal cut-off for IgG was 95.70 mg/dL, with a sensitivity of 76%, specificity of 94%, and an AUC of 0.88, using PCR results as the gold standard. Conclusions: This study emphasizes the importance of accurately diagnosing H. pylori infections, highlighting that PCR offers greater sensitivity and specificity than ELISA. By establishing optimal IgA and IgG cut-off points, the study enhances diagnostic precision, which could lead to improved therapeutic interventions and patient outcomes. | en |
| DOI | https://doi.org/10.5812/archcid-150644 | en |
| Keyword | Diagnostic Accuracy | en |
| Keyword | ELISA | en |
| Keyword | PCR | en |
| Keyword | <i>Helicobacter pylori</i> | en |
| Keyword | Gastric Problems | en |
| Publisher | Brieflands | en |
| Title | Diagnostic Accuracy of Enzyme-Linked Immunosorbent Assay Compared with Polymerase Chain Reaction in Diagnosing <i>Helicobacter pylori</i> in Patients with Gastric Problems | en |
| Type | Research Article | en |
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