Development and Evaluation of a Real-Time TaqMan-PCR for the Detection of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients
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Introduction: Cytomegalovirus (CMV) has been recognized as the most important viral pathogen in persons undergoing
bone marrow transplantation (BMT). In this study, we present the development of a TaqMan-based real-time PCR
assay to quantify human cytomegalovirus (CMV) DNA in peripheral blood leukocytes (PBLs) of bone marrow transplantation
patients.