Determination of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC

Abstract

A high performance liquid chromatography method was studied for determination of 18 β-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and UV detection at 230 nm. The recoveries were %99.60-%102.81 with relative standard deviation between %0.01-%1.58. The relative standard deviation of the repeatability test was %2.96. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products.