In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein
| Author | Hamid Abtahi | en |
| Author | Leila Farhangnia | en |
| Author | Ehsanollah Ghaznavi-Rad | en |
| Orcid | Ehsanollah Ghaznavi-Rad [0000-0002-8484-7166] | en |
| Issued Date | 2016-03-01 | en |
| Abstract | Background: Bacterial infection by antibiotic-resistant Staphylococcus aureus strains is a worldwide concern and the development of novel antistaphylococcal agents is acutely needed. Lysostaphin, an example of such novel agents, is a bacteriocin secreted by S. simulans to kill S. aureus through proteolysis of the Staphylococcus cell wall. Objectives: The aim of this study was to evaluate the in vitro and in vivo antistaphylococcal activity of recombinant lysostaphin. Materials and Methods: The in vitro study of the recombinant lysostaphin activity against S. aureus was determined by turbidimetric assay. For in vivo investigation, two groups of rats were inoculated with 1.4 × 109 CFU S. aureus. Five days after the nasal instillation of S. aureus, treatment in one of the groups was performed with a single dose (200 μg/dose) of recombinant lysostaphin formulated in Eucerin-based cream. Results: Recombinant lysostaphin at 100 μg/mL concentration showed a significant decrease of the optical density compared to the control samples. The in vivo study demonstrated that a single dose (200 μg/dose) of recombinant lysostaphin cream significantly reduced nasal colonization in all the treated animals compared to the untreated ones. Conclusions: These results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal S. aureus in rats. It can be recommended for human clinical trial studies. | en |
| DOI | https://doi.org/10.5812/jjm.28489 | en |
| Keyword | Lysostaphin | en |
| Keyword | Recombinant Protein | en |
| Keyword | <i>Staphylococcus aureus</i> | en |
| Publisher | Brieflands | en |
| Title | In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein | en |
| Type | Research Article | en |
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