Quercetin and Catechin Modulate Alloxan-Induced Nephrotoxicity in Mice by Affecting Oxidative Stress, Inflammation and COX-2 Protein Expression
| Author | Amir Hossein Rastineh | en |
| Author | Maryam Khamisi Pour | en |
| Author | Ali Vadizadeh | en |
| Author | Masoud Mahdavinia | en |
| Author | Fereshtesadat Fakhredini | en |
| Author | Mahdieh Sadat Badiee | en |
| Orcid | Masoud Mahdavinia [0000-0001-9448-1500] | en |
| Orcid | Fereshtesadat Fakhredini [0000-0002-7766-3369] | en |
| Orcid | Mahdieh Sadat Badiee [0000-0001-7405-5950] | en |
| Issued Date | 2026-02-28 | en |
| Abstract | Background: Nephrotoxicity is defined as the swift deterioration of kidney function resulting from the harmful impacts of drugs and chemicals. Alloxan (ALLO) triggers the activation of oxidative and inflammatory factors, contributing to nephrotoxicity. Quercetin (QCT) and catechin (CAT) play a crucial role in both the treatment and prevention of disease-related complications, owing to their antioxidant characteristics. Objectives: The aim of this research was to evaluate the combined effects of QCT and CAT on ALLO-induced nephrotoxicity in mice by affecting oxidative stress, inflamation, and COX-2 protein expression. Methods: A total of thirty male mice were divided into five groups of six with a power analysis, then randomization was performed using computer-generated sequences and assignment concealment. The current research was supported by the Toxicology Research Center, Medical Basic Sciences Research Institute of the AJUMS Foundation in Iran (03s53). The group classification was: Control (10 mL/kg normal saline); ALLO (120 mg/kg, i.p.); ALLO+QCT (150 mg/kg, gavage); ALLO+CAT (150 mg/kg, gavage); and ALLO+QCT+CAT. Alloxan was administered via injection every other day for a total of six days. Subsequently, QCT and CAT were administered to the mice through gavage for a duration of 14 days. After a period of 21 days and one night of fasting, the mice were anesthetized, and blood was collected from their hearts while their kidneys were extracted. The levels of biochemical markers, oxidative stress, inflammation factors, and the protein expression of cyclooxygenase-2 (COX-2) were assessed, and histological studies were conducted. Results: Administration of QCT and CAT resulted in a decrease in the serum levels of fasting blood sugar (FBS), cholesterol (Cho), triglycerides (TGs), low-density lipoprotein, uric acid (U.A), blood urea nitrogen (BUN), and creatinine (Cr), and an increase in the level of high-density lipoprotein compared with the ALLO group (P < 0.001). The levels of tissue factors total thiol, catalase, and superoxide dismutase (SOD) increased in the QCT and CAT groups compared with the ALLO group (P < 0.001), and the levels of thiobarbituric acid-reactive substances, reactive oxygen species (ROS), advanced oxidation protein products (AOPP), tumor necrosis factor alpha, and COX-2 protein expression decreased in the QCT and CAT groups compared with the ALLO group (P < 0.001). Conclusions: The management of QCT and CAT may influence ALLO-induced nephrotoxicity in mice by reducing oxidative stress, inflammation, and the expression of COX-2 protein. | en |
| DOI | https://doi.org/10.5812/jjnpp-167250 | en |
| Keyword | Alloxan | en |
| Keyword | Quercetin | en |
| Keyword | Catechin | en |
| Keyword | Nephrotoxicity | en |
| Keyword | COX-2 | en |
| Keyword | Mice | en |
| Publisher | Brieflands | en |
| Title | Quercetin and Catechin Modulate Alloxan-Induced Nephrotoxicity in Mice by Affecting Oxidative Stress, Inflammation and COX-2 Protein Expression | en |
| Type | Research Article | en |