Evaluation of binding of FITC-prolactin conjugate to prolactin receptor
| Author | Majid sirati Sabet | en |
| Author | F Karami Tehrani | en |
| Issued Date | 2007-03-31 | en |
| Abstract | ¯Abstract Background: Prolactin is an important mammalian hormone and the associated receptor is recognized in many different cells. Radioligand and histochemical methods are both used for assaying prolactin receptor. Objective: To produce FITC-prolactin conjugate and also to study the ability of conjugate to bind prolactin receptor. Methods: In this experimental study FITC was bound to prolactin in alkaline solution. FITC-prolactin conjugate was separated from free FITC by chromatographic method. Later, the ability of FITC-prolactin conjugate to bind prolactin receptor of peripheral blood mononuclear cells (PBMC) was assessed by flowcytometry. Findings: Fluorescence emission was detected in 2.1% of the cells in absence of FITC-prolactin. Following the addition of FITC-prolactin conjugate to cells for one hour and further washing, the fluorescence emission was detected in 27.8% of cells. For PBMC, these data were 0.02% and 11.8%, respectively. Conclusion: Regarding the data obtained in our study, FITC-prolactin conjugate can bind prolactin receptor. Therefore, this conjugate could be used for assessing prolactin receptor by fluorometric method. | en |
| DOI | https://doi.org/ | en |
| Keyword | Keywords: Prolactin | en |
| Keyword | Prolactin Receptor | en |
| Keyword | FITC | en |
| Keyword | Peripheral Blood Mononuclear Cells | en |
| Keyword | Prolactin Conjugate | en |
| Keyword | Flowcytometry | en |
| Publisher | Brieflands | en |
| Title | Evaluation of binding of FITC-prolactin conjugate to prolactin receptor | en |
| Type | Research Article | en |
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