Multiple-Locus Variable-Number Tandem Repeat Analysis Genotyping of Biofilm-Producing <i>Pseudomonas aeruginosa</i> Clinical Isolates
| Author | Raziyeh Ramazani | en |
| Author | Rabeeh Izadi Amoli | en |
| Author | Mojtaba Taghizadeh Armaki | en |
| Author | Abazar Pournajaf | en |
| Author | Hami Kaboosi | en |
| Orcid | Mojtaba Taghizadeh Armaki [0000-0003-1811-5829] | en |
| Orcid | Abazar Pournajaf [0000-0002-3407-0950] | en |
| Orcid | Hami Kaboosi [0000-0002-3407-0950] | en |
| Issued Date | 2024-03-31 | en |
| Abstract | Background: Pseudomonas aeruginosa significantly contributes to hospital-acquired infections. Objectives: This study aimed to investigate the genetic diversity of P. aeruginosa strains using multiple-locus variable-number tandem repeat analysis (MLVA) and to explore the relationship between biofilm production and antibiotic resistance. Methods: In this cross-sectional study, 79 P. aeruginosa isolates were collected. Antibiotic sensitivity was tested using the Kirby-Bauer method, and biofilm production capability was assessed through the microtiter plate method. Genetic diversity was evaluated by MLVA, analyzing eight variable-number tandem repeat (VNTR) loci: MS-213, MS-214, MS-207, MS-217, MS-222, MS-209, MS-77, and MS-172. Phylogenetic relationships were delineated using PHYLOViZ 2.0 software. Results: The patient cohort comprised 51.9% males, with the majority of samples (35.4%) obtained from urine. Ceftazidime (CAZ 30µg) showed the highest resistance rate at 77.2%. Notably, 92.4% of isolates were capable of forming biofilms, categorized as 22.7% weak, 28.7% moderate, and 46.5% strong. Phylogenetic analysis demonstrated variability across one or more VNTR loci. Simpson’s index (0.906) and Shannon-Weiner diversity indices (H: 3.466, J: 0.910, Hmax: 3.807, Hmin: 1.242) identified MS77 as the most informative marker for genetic diversity among the isolates. Conclusions: The study highlights an alarming trend in antibiotic resistance, underscoring the necessity of regular monitoring. The findings confirm that MLVA is a straightforward, rapid genotyping method suitable for assessing the genetic diversity of P. aeruginosa. | en |
| DOI | https://doi.org/10.5812/jjm-143820 | en |
| Keyword | <i>Pseudomonas aeruginosa</i> | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | Biofilm | en |
| Keyword | VNTR | en |
| Keyword | MLVA | en |
| Publisher | Brieflands | en |
| Title | Multiple-Locus Variable-Number Tandem Repeat Analysis Genotyping of Biofilm-Producing <i>Pseudomonas aeruginosa</i> Clinical Isolates | en |
| Type | Research Article | en |