Chrysin and Gallic Acid Protect the Hippocampal Neurons and Mitigate Blood-Brain Barrier Disruption in a Mouse Model of Global Cerebral Ischemia
| Author | Ali Vakili | en |
| Author | Shahein Momenabadi | en |
| Author | Ahmad Reza Bandegi | en |
| Author | Abbas Ali Vafaei | en |
| Author | Abedin Vakili | en |
| Orcid | Ali Vakili [0000-0002-5761-7904] | en |
| Orcid | Shahein Momenabadi [0000-0002-5844-5921] | en |
| Orcid | Ahmad Reza Bandegi [0000-0003-1834-0583] | en |
| Orcid | Abbas Ali Vafaei [0000-0003-1178-3787] | en |
| Orcid | Abedin Vakili [0000-0002-4269-9978] | en |
| Issued Date | 2025-02-28 | en |
| Abstract | Background: Cerebral ischemia activates harmful biochemical pathways that result in blood-brain barrier (BBB) breakdown and neuronal damage. Natural compounds such as chrysin and gallic acid (GA), known for their antioxidant and anti-inflammatory properties, may protect the BBB and reduce neuronal injury. Objectives: This study aimed to examine the effects of combining chrysin and GA on hippocampal neuronal damage, cognitive function, BBB integrity, and claudin-5 expression in a mouse model of cerebral ischemia. Methods: Cerebral ischemia was induced through bilateral common carotid artery occlusion (BCCAO) for 30 minutes, followed by 48 hours of reperfusion. Chrysin (30 mg/kg, intraperitoneally), GA (50 mg/kg, intraperitoneally), and their combination were administered at the start of reperfusion and subsequently at 30 minutes and 1 hour. Hippocampal neuronal damage, spatial memory, Evans blue (EB) leakage, and claudin-5 expression were evaluated 48 hours after reperfusion. Results: Administration of chrysin, GA, and their combination significantly enhanced neuronal survival in the CA1, CA3, and dentate gyrus (DG) regions (P < 0.001). The combination diminished neurological deficit scores (1.5 ± 0.22 vs. control 3.5 ± 0.56, P < 0.05) and escape latency time (12.8 ± 4.5 vs. control 40 ± 4.82 seconds, P < 0.01). Likewise, these interventions significantly reduced EB leakage (3.46 ± 0.62 vs. control 11.28 ± 0.98 μg/g of brain tissue) and upregulated claudin-5 expression (38% ± 1.29 vs. control 10.75% ± 1.65, P < 0.001). Conclusions: This study demonstrated that the combined treatment of chrysin and GA synergistically promoted hippocampal neuron survival, improved neurological function, and maintained BBB integrity by upregulating claudin-5 expression. We suggest that this therapeutic approach may offer potential benefits for stroke patients, though further experimental and clinical investigation is required to confirm its efficacy. | en |
| DOI | https://doi.org/10.5812/jjnpp-159908 | en |
| Keyword | Chrysin | en |
| Keyword | Gallic Acid | en |
| Keyword | Combination | en |
| Keyword | Brain Ischemia | en |
| Keyword | Claudin-5 | en |
| Keyword | BBB | en |
| Keyword | Mice | en |
| Publisher | Brieflands | en |
| Title | Chrysin and Gallic Acid Protect the Hippocampal Neurons and Mitigate Blood-Brain Barrier Disruption in a Mouse Model of Global Cerebral Ischemia | en |
| Type | Research Article | en |
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