Evaluation of Ascorbic Acid Niosomes as Potential Detoxifiers in Oxidative Stress-induced HEK-293 Cells by Arsenic Trioxide
Author | Akram Oftadeh Harsin | en |
Author | Farzin Firozian | en |
Author | Meysam Soleimani | en |
Author | Fereshteh Mehri | en |
Author | Akram Ranjbar | en |
Orcid | Akram Oftadeh Harsin [0000-0001-6485-5794] | en |
Orcid | Farzin Firozian [0000-0003-4794-8838] | en |
Orcid | Meysam Soleimani [0000-0003-2505-8834] | en |
Orcid | Fereshteh Mehri [0000-0003-0106-245X] | en |
Orcid | Akram Ranjbar [0000-0002-4564-3603] | en |
Issued Date | 2022-12-31 | en |
Abstract | Background: As an environmental contaminant, Arsenic (As) poses many risks to human health. Increased Oxidative Stress (OS) and decreased antioxidant cell defense are the suggested mechanisms of carcinogenicity and toxicity of As. As a powerful antioxidant and water-soluble compound, vitamin C protects cells and tissues against oxidation and has a wide range of healing properties. Objectives: The current study aimed to formulate a suitable ascorbic acid (vitamin C) niosome and compare it with vitamin C in preventing As-induced toxicity in HEK-293 cells. Methods: Various formulas of vitamin C niosomes were prepared by C-SPAN mixed with cholesterol. The physicochemical characteristics of niosomal formulations, including load size, zeta-potential, and the drug release profile, were evaluated in HEK-293 cells. Then, OS biomarkers such as total reactive oxygen species (ROS), malondialdehyde (MDA), catalase (CAT), Antioxidant Capacity (TAC), and superoxide dismutase (SOD) activities determined the protective effects of vitamin C niosomes compared with vitamin C against As-induced toxicity. Results: The particle size and zeta potential of the optimal vitamin C niosome were 163.2 ± 6.1 nm and 23.3 ± 3.5 mV, respectively. Arsenic increased ROS and MDA levels while decreasing CAT, TAC, and SOD activities in the HEK-293 cell line. Finally, the vitamin C niosome decreased OS and increased antioxidant properties more than vitamin C. Significance: Vitamin C niosome was more effective than vitamin C in treating As-induced toxicity in vitro. | en |
DOI | https://doi.org/10.5812/ijpr-127038 | en |
Keyword | Vitamin C | en |
Keyword | Arsenic | en |
Keyword | Niosome | en |
Keyword | Oxidative Stress | en |
Keyword | HEK-293 | en |
Publisher | Brieflands | en |
Title | Evaluation of Ascorbic Acid Niosomes as Potential Detoxifiers in Oxidative Stress-induced HEK-293 Cells by Arsenic Trioxide | en |
Type | Research Article | en |