MicroRNA-642a-5p Targets DNA Damage-Inducible Transcript 4 to Suppress Hepatitis B Virus Hepatoma Carcinoma Cell
| Author | Min Ding | en |
| Author | Juan Yang | en |
| Author | XueLi Zeng | en |
| Author | Pei Liu | en |
| Author | ShunLing Zhang | en |
| Author | Sheng Zheng | en |
| Orcid | Min Ding [0009-0007-1118-2259] | en |
| Orcid | Juan Yang [0009-0000-5096-4324] | en |
| Orcid | XueLi Zeng [0009-0000-7645-2212] | en |
| Orcid | Pei Liu [0009-0003-6920-1812] | en |
| Orcid | ShunLing Zhang [0009-0007-4878-4632] | en |
| Orcid | Sheng Zheng [0009-0009-5511-7410] | en |
| Issued Date | 2024-03-31 | en |
| Abstract | Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors in clinical practice, with hepatitis B virus (HBV) being the most common risk factor for HCC. MicroRNAs (miRNAs) have emerged as a new marker for disease diagnosis and molecularly targeted therapies; however, the mechanism of miR-642a-5p in HBV-associated HCC remains unclear. Objectives: The aim of this study was to investigate the expression of miR-642a-5p, which targets DNA damage-inducible transcript 4 (DDIT4), in HBV-associated HCC, and its effect on the proliferation, migration, and invasion of HBV-positive HCC cells. Methods: miR-642a-5p in the serum of patients with HBV-associated liver cancer (LC), as well as miR-642a-5p and DDIT4 mRNA in LC tissues and cells, and HBV DNA in HBV-positive cells were detected. The targeting of DDIT4 by miR-642a-5p and the progression of cells were also examined. All cell experiments were repeated five times. Results: The results indicated that levels of miR-642a-5p were decreased, while levels of DDIT4 were increased in the serum, tissues, and cells of HBV-positive HCC patients. Overexpression of miR-642a-5p inhibited the progression of HBV-positive HCC cells, suppressed HBV DNA replication, cell proliferation, and invasion, and promoted apoptosis in HepG2.2.15 cells. Conclusions: In addition, miR-642a-5p directly targeted DDIT4, and knockdown of DDIT4 reversed the effects of miR-642a-5p upregulation, promoting the progression of HBV-positive HCC cells. In conclusion, miR-642a-5p is expressed at low levels in HBV-associated HCC and inhibits HBV DNA replication and tumor progression in HBV-positive HCC by targeting DDIT4. This study provides a foundation for molecular targeted therapy in HBV-positive HCC. | en |
| DOI | https://doi.org/10.5812/jjm-145798 | en |
| Keyword | Hepatitis B Virus | en |
| Keyword | Liver Cancer | en |
| Keyword | MicroRNA-642a-5p | en |
| Keyword | DNA Damage-Inducible Transcript 4 | en |
| Publisher | Brieflands | en |
| Title | MicroRNA-642a-5p Targets DNA Damage-Inducible Transcript 4 to Suppress Hepatitis B Virus Hepatoma Carcinoma Cell | en |
| Type | Research Article | en |