Molecular Analysis and Integron Carriage of Mupirocin-Resistant <i>Staphylococcus aureus</i> Strains Isolated from Burn Wound Infections, Tehran, Iran
| Author | Masoumeh Navidinia | en |
| Author | Hossein Goudarzi | en |
| Author | Ramin Pouriran | en |
| Author | Hadi Azimi | en |
| Author | Mehdi Goudarzi | en |
| Orcid | Masoumeh Navidinia [0000-0001-7124-9573] | en |
| Orcid | Mehdi Goudarzi [0000-0001-6720-9341] | en |
| Issued Date | 2019-02-18 | en |
| Abstract | Background: The prevalence of wound infections associated with multidrug-resistant Staphylococcus aureus (S. aureus) strains among burns patients has tremendously increased. Recently, the role of integrons as a key system involved in spreading multi-drug resistance has been well documented. Objectives: To investigate the prevalence of integrons, antimicrobial resistance pattern, and S. aureus protein A (spa) typing of the mupirocin-resistant S. aureus (MRSA) strains obtained from a referral burns hospital in Tehran, Iran. Methods: This study was performed during January 2017 to April 2018 on 64 MRSA isolates gathered from burns patients. The isolates were examined for susceptibility to antimicrobial agents. The integrase-encoding gene was detected by conventional polymerase chain reaction (PCR). Integrase-positive strains were evaluated for the determination of integron classes by using PCR-restriction fragment length polymorphism assay, and genotyping was performed by spa typing. Results: Out of 64 MRSA strains, 24 (37.5%) and 40 (62.5%) isolates carried high-level and low-level resistance, respectively. The findings revealed that the entire isolates were MRSA. They belonged to six different spa types including t860 (40.6%), t790 (21.8%), t037 (17.2%), t064 (17.2%), t008 (1.6%), and t631 (1.6%). Class 1 and 2 integrons were found in 59.4% and 17.2% of the isolates, respectively, and 4 (6.2%) isolates were observed to carry class 1 and 2 integrons simultaneously. Conclusions: Precise epidemiological monitoring of integrons is required to prevent mupirocin resistance dissemination in MRSA strains. | en |
| DOI | https://doi.org/10.5812/archcid.84764 | en |
| Keyword | Mupirocin | en |
| Keyword | Polymerase Chain Reaction | en |
| Keyword | Methicillin-Resistant <i>S. aureus</i> | en |
| Keyword | <i>Spa</i> typing | en |
| Publisher | Brieflands | en |
| Title | Molecular Analysis and Integron Carriage of Mupirocin-Resistant <i>Staphylococcus aureus</i> Strains Isolated from Burn Wound Infections, Tehran, Iran | en |
| Type | Research Article | en |
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