<i>In Vitro</i> Activity of Two Nanoparticles on Clinical Isolates of <i>Candida parapsilosis</i>, Showing Resistance Against Antifungal Agents in Children

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AuthorEnsieh Lotfalien
AuthorAhmad Reza Shahverdien
AuthorRasoul Mohammadien
AuthorFatemeh Noorbakhshen
AuthorAli Ghajarien
AuthorSaham Ansarien
AuthorSassan Rezaieen
OrcidEnsieh Lotfali [0000-0001-6709-1320]en
OrcidRasoul Mohammadi [0000-0002-8220-4511]en
OrcidSassan Rezaie [0000-0001-6709-1320]en
Issued Date2017-10-31en
AbstractBackground: Resistance of pathogenic fungi to conventionally available antifungal agents has been increasing and has become a serious problem. On the other hand, nanoscience has emerged as a powerful tool capable of developing and designing new antimicrobial drugs. Objectives: The aim of this study was to test minimum inhibitory concentrations (MICs) of silver (Ag-NPs) and selenium (Se-NPs) nanoparticles, both alone and in combination with antifungal agents fluconazole (FLU), itraconazole (ITR), and amphotericin B (AMB) on resistant C. parapsilosis strains. Methods: Eight C. parapsilosis strains resistant to antifungal agents (FLU, ITR, AMB) and the standard strain of C. parapsilosis (ATCC 22019) as a control were used in this study. The standard protocol of antifungal susceptibility testing for yeast was performed by the broth micro-dilution method described in the clinical and laboratory standards institute (CLSI) guidelines, document M 27-S4. Results and Conclusions: MICs of standard and resistant strains were different against nano compounds, both alone and in combination with antifungal drugs. The growth of yeast was inhibited by Ag-NPs at concentrations as low as 2 µg /mL (against FLCR1 and FLCR3) and 0.25 µg /mL (against ITRR2 and AMBR2). Antifungal activity testing of Se-NPs revealed that it can inhibit the growth of yeasts at concentrations as low as 4 µg /mL (against FLCR1 and FLCR3) and 0.5 mg/mL (against ITRR2, ITRR3 and AMBR1). The results demonstrated that Se-NPs had lower activity than Ag-NPs, but both (Se-NPs and Ag-NPs) had higher activity than common antifungal agents. Further in vitro and in vivo studies are required for this.en
DOIhttps://doi.org/10.5812/archcid.13853en
KeywordNanoparticlesen
KeywordFungal Drug Sensitivity Testsen
Keyword<i>Candida parapsilosis</i>en
PublisherBrieflandsen
Title<i>In Vitro</i> Activity of Two Nanoparticles on Clinical Isolates of <i>Candida parapsilosis</i>, Showing Resistance Against Antifungal Agents in Childrenen
TypeResearch Articleen

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