?-Lactamase Typing by Substrate Hydrolysis in Clinical Isolates of Methicillin Resistant Staphylococcus Aureus
| Author | Katayoun Borhani | en |
| Author | Fereshteh Eftekhar | en |
| Author | Somayeh Danesh Monfared | en |
| Issued Date | 2009-10-31 | en |
| Abstract | Background: Staphylococcus aureus is one of the most important causes of nosocomial infections and most clinical isolates are multidrug resistant. Resistance to ?-lactam antibiotics is most often due to bacterial ?-lactamase production. Characterization of ?-lactamases is important for choosing appropriate antibiotic therapy. Materials and Methods: Thirty methicillin resistant Staphylococcus aureus (MRSA) were identified by standard biochemical methods. Antibacterial susceptibility to 9 ?-lactam antibiotics was determined. ?-lactamase production was shown in all isolates using the colony iodometric test and nitrocefin discs. ?-lactamase typing was carried out by measuring the relative substrate hydrolysis rates. Results: The MRSA isolates were resistant to the majority of ?-lactam antibiotics. The results showed that 90% of the isolates displayed type A substrate hydrolysis profile of ?-lactamase. Conclusion: The alarming high level of resistance to ?-lactam antibiotics including methicillin and 3rd generation ?-lactams show the need for extensive studies on alternative treatment protocols and use of new drugs | en |
| DOI | https://doi.org/ | en |
| Keyword | Methicillin resistant Staphylococcus aureus (MRSA) | en |
| Keyword | Antibiotic resistance | en |
| Keyword | ?-lactamase typing | en |
| Publisher | Brieflands | en |
| Title | ?-Lactamase Typing by Substrate Hydrolysis in Clinical Isolates of Methicillin Resistant Staphylococcus Aureus | en |
| Type | Research Article | en |
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