Role of Toll-Like Receptors in Tuberculosis Infection
| Author | Oguz Oben Biyikli | en |
| Author | Aysegul Baysak | en |
| Author | Gulfem Ece | en |
| Author | Adnan Tolga Oz | en |
| Author | Mustafa Hikmet Ozhan | en |
| Author | Afig Berdeli | en |
| Issued Date | 2016-10-01 | en |
| Abstract | Background: One-third of the world’s population is infected with Mycobacterium tuberculosis. Investigation of Toll-like receptors (TLRs) has revealed new information regarding the immunopathogenesis of this disease. Toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. Toll-like receptor 2 and TLR-4 have been identified in association with tuberculosis infection. Objectives: The aim of our study was to investigate the relationship between TLR polymorphism and infection progress. Methods: Twenty-nine patients with a radiologically, microbiologically, and clinically proven active tuberculosis diagnosis were included in this 25-month study. Toll-like receptor 2 and TLR-4 polymorphisms and allele distributions were compared between these 29 patients and 100 healthy control subjects. Peripheral blood samples were taken from all patients. Genotyping of TLR-2, TLR-4, and macrophage migration inhibitory factor was performed. The extraction step was completed with a Qiagen mini blood purification system kit (Qiagen, Ontario, Canada) using a peripheral blood sample. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. Results: In total, 19 of the 29 patients with tuberculosis infection had a TLR-2 polymorphism, and 20 of the 100 healthy subjects had a TLR-2 polymorphism (P < 0.001). The TLR-4 polymorphism and interferon-γ allele distributions were not statistically correlated. Conclusions: Toll-like receptor 2 polymorphism is a risk factor for tuberculosis infection. The limiting factor in this study was the lack of investigation of the interferon-γ and tumor necrosis factor-α levels, which are important in the development of infection. Detection of lower levels of these cytokines in bronchoalveolar lavage specimens, especially among patients with TLR-2 defects, will provide new data that may support the results of this study. | en |
| DOI | https://doi.org/10.5812/jjm.20224 | en |
| Keyword | Toll-Like Receptor | en |
| Keyword | Infection | en |
| Keyword | Genetic Polymorphism | en |
| Keyword | <i>Mycobacterium tuberculosis</i> | en |
| Publisher | Brieflands | en |
| Title | Role of Toll-Like Receptors in Tuberculosis Infection | en |
| Type | Research Article | en |
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