Genetic Determinants of Antibiotic Resistance in Hospital and Community Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>
| Author | Farzaneh Dehghan | en |
| Author | Nader Zolghadri | en |
| Author | Afsaneh Karmostaji | en |
| Orcid | Afsaneh Karmostaji [0000-0002-6273-7812] | en |
| Issued Date | 2017-05-31 | en |
| Abstract | Background: Multi-drug resistant Escherichia coli and Klebsiella pneumoniae with various resistance determinants are a major concern in hospital and community acquired infections around the world. Objectives: To describe the presence of blaCTX-M, blaTEM, blaPER, blaVEB, and integrons class 1, 2, 3 and extended spectrum β lactamase (ESBL) phenotype in E. coli and K. pneumoniae isolates from clinical samples of inpatients and outpatients. Methods: One hundred and eighty six E. coli and fifty-eight K. pneumoniae were collected. Antimicrobial susceptibility test was performed by disk diffusion method. Extended-spectrum beta-lactamase phenotype were screened by phenotypic confirmatory test. PCR assay was performed for blaTEM, blaCTX-M, blaPER and blaVEB and class 1, 2, 3 integrase genes. Statistical analysis was performed by chi-squared test. Results: Extended-spectrum beta-lactamase phenotype was detected in 49 (26.3%) E. coli and 19 (32.8%) K. pneumoniae isolates. BlaVEB gene in 32 (17.2%) E. coli and 5 (8.6%) K. pneumoniae isolates. BlaPER gene in 4 (2.1%) E. coli and 0 (0%) K. pneumoniae isolates. BlaCTX-M gene in 113 (60.7%) E. coli and 34 (58.6%) K. pneumoniae isolates. BlaTEM gene in 106 (57%) E. coli and 25 (43.1%) K. pneumoniae isolates. One hundred and nine (58.6%) of E. coli and 33 (56.9%) of K. pneumoniae were carrying Class 1 integron and 18 (9.7%) of E. coli and 3 (5.2%) of K. pneumoniae were carrying Class 2 integron. Class 3 integron was not detected. Conclusions: High prevalence of ESBLs in E. coli and K. pneumoniae isolated from the community and hospital acquired infections could lead to the wide spread of multi-drug resistance clones that also contain new mechanism of resistance. | en |
| DOI | https://doi.org/10.5812/jjm.45678 | en |
| URI | https://brieflands.com/journals/jjm/articles/13513 | en |
| Keyword | Community-Acquired Infection | en |
| Keyword | β Lactamases | en |
| Keyword | Hospital Infections | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | Integrons | en |
| Publisher | Brieflands | en |
| Title | Genetic Determinants of Antibiotic Resistance in Hospital and Community Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i> | en |
| Type | Research Article | en |
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