The Dominance of Pilus Islet 1 in Pneumococcal Isolates Collected From Patients and Healthy Individuals
| Author | Farzaneh Khodaei | en |
| Author | Ali Ahmadi | en |
| Author | Shirin Sayahfar | en |
| Author | Gholamreza Irajian | en |
| Author | Malihe Talebi | en |
| Orcid | Malihe Talebi [0000-0001-9630-1083] | en |
| Issued Date | 2016-05-01 | en |
| Abstract | Background: Pili in Streptococcus pneumoniae have been shown to be one of the adherence factors for epithelial cells in the human upper respiratory tract. Two types of pilus-like structures (pilus islet-1 and pilus islet-2) have been distinguished in S. pneumoniae. Objectives: To investigate the presence of pilus islet-1 (PI-1) in S. pneumoniae and the correlation between our isolates. Materials and Methods: In this study, 162 S. pneumoniae isolates were collected from clinical specimens, and normal flora were also examined for the distribution of PI-1 using the presence of the rlrA and rrgC genes as markers for this islet and sipA as an indicator of pilus islet-2 (PI-2). BOX-PCR analyses were performed to determine the genetic relationship between isolates. Results: The results confirmed the presence of rlrA and rrgC genes in both clinical (n = 39) and normal flora (n = 26) isolates. The minimal inhibitory concentration results revealed that the rate of resistance of these isolates to the three antibiotics tested ranged from 26% for penicillin to 46% for erythromycin and tetracycline. Furthermore, 12% of the isolates were resistant to all three antibiotics. Strain typing using repetitive element BOX-PCR analysis among the 65 isolates identified 8 different band patterns. Conclusions: Our results indicated that the dissemination of PI-1 was widespread in S. pneumoniae isolates, although no PI-2 isolates were detected. Furthermore, the frequency of rlrA and rrgC of clinical isolates was significantly more than that of normal flora isolates. | en |
| DOI | https://doi.org/10.5812/jjm.30470 | en |
| Keyword | Pilus | en |
| Keyword | BOX-PCR | en |
| Keyword | <i>Streptococcus pneumonia</i> | en |
| Publisher | Brieflands | en |
| Title | The Dominance of Pilus Islet 1 in Pneumococcal Isolates Collected From Patients and Healthy Individuals | en |
| Type | Research Article | en |