Role of HDM2 Gene in Radio-Sensitivity of Esophageal Cancer Cell Lines to Irradiation
| Author | Mohsen Mohammadi | en |
| Author | Jalil Pirayesh Islamian | en |
| Author | Hadi Karami | en |
| Author | Maryam Oladghaffari | en |
| Author | Alireza Farajollahi | en |
| Author | Kazem Nejati-Koshki | en |
| Issued Date | 2017-06-30 | en |
| Abstract | Background: Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients. Objectives: Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells. Methods: The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines. Results: It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines. Conclusions: Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and genome protection Cell cycle control, programmed cell death and response cells to radition play important roles. | en |
| DOI | https://doi.org/10.5812/ijcm.8950 | en |
| Keyword | Esophagus Cancer | en |
| Keyword | Hdm2 | en |
| Keyword | P53 | en |
| Keyword | Radiosensitivity | en |
| Keyword | Squamous Cell Carcinoma | en |
| Publisher | Brieflands | en |
| Title | Role of HDM2 Gene in Radio-Sensitivity of Esophageal Cancer Cell Lines to Irradiation | en |
| Type | Research Article | en |
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