Sensitivity of Susceptibility-Weighted Imaging in Detecting Superparamagnetic Iron Oxide-Labeled Mesenchymal Stem Cells: A Comparative Study
| Author | Serah Park | en |
| Author | Byung Kook Kwak | en |
| Author | Jisung Jung | en |
| Orcid | Byung Kook Kwak [0000-0003-1550-9291] | en |
| Issued Date | 2015-04-01 | en |
| Abstract | Background: Susceptibility-weighted imaging (SWI) is extremely sensitive in the detection of superparamagnetic iron oxide (SPIO) nanoparticle-labeled cells. However, no study has compared molecular imaging for stem cell detection using SWI and other MRI pulse sequences. Objectives: This study aims to assess the sensitivity of SWI in detecting SPIO nanoparticle-labeled, human bone marrow-derived mesenchymal stem cells (SPIO-hMSCs) compared with that of T2- and T2*-weighted imaging (T2WI and T2*WI, respectively) in a phantom and in vivo study in rats. Materials and Methods: A phantom was prepared with various cell concentrations. In one normal rat, SPIO-hMSCs were implanted directly through burr holes into both caudate putamens, while in three rats without and six rats with photothrombotic infarction, 2.5 × 105/ml SPIO-hMSCs were infused into the ipsilateral internal carotid artery (ICA). T2WI, T2*WI, and SWI findings were compared for dark regions representing SPIO-hMSCs. Results: SWI and T2*WI detected 15 µL of 13 SPIO-hMSCs/µL and 15 µL of 27 SPIO-hMSCs/µL in the phantom, respectively and 3 µL of 333 SPIO-hMSCs/µL and 3 µL of 167 SPIO-hMSCs/µL in the normal rat brain (direct implantation). In the normal rat brain (ICA infusion), one of the three cases showed numerous foci of dark regions dispersed throughout the brain on T2*WI and SWI. Dark regions surrounded the infarcts in all six infracted rat brains. The dark region was most prominent on SWI, followed by T2*WI and T2WI in all six rats (P = 0.002). Implanted SPIO-hMSCs were confirmed using Prussian blue staining. Conclusions: SWI is the most sensitive in the detection of SPIO-hMSCs, with the dark regions representing SPIO-hMSCs being more prominent on SWI than on T2*WI and T2WI. | en |
| DOI | https://doi.org/10.5812/iranjradiol.20782 | en |
| Keyword | Mesenchymal Stromal Cells | en |
| Keyword | Cerebral Infarction | en |
| Keyword | Rats | en |
| Keyword | Ferrosoferric Oxide | en |
| Publisher | Brieflands | en |
| Title | Sensitivity of Susceptibility-Weighted Imaging in Detecting Superparamagnetic Iron Oxide-Labeled Mesenchymal Stem Cells: A Comparative Study | en |
| Type | Research Article | en |