Molecular Epidemiology of AmpC-Producing Klebsiella Pneumoniae Isolated from Perioperative Patients in a Tertiary Hospital
| Author | Roghayeh Gholizadeh Doran Mahalleh | en |
| Author | Nadia Kazemi Pour | en |
| Author | Farokh Rokhbakhsh-Zamin | en |
| Issued Date | 2021-09-30 | en |
| Abstract | Background: Extended-spectrum cephalosporin-resistant organisms are the sources of AmpC- type ?-lactamase isolates. AmpC-producing Klebsiella pneumoniae isolates are a major challenge in clinics due to their high rate of antibiotic resistance. This study aimed to detect AmpC ?-lactamases in K. pneumoniae isolated from hospitalized patients with a surgical history. Materials and Methods: This cross-sectional study was performed on 120 urine catheter samples. After antibiotic susceptibility testing, plasmid-mediated AmpC was detected by double-disc plus combined disc test (DCDT) and three-dimensional extract test (TDET). The genotypic detection of plasmid-mediated AmpC was carried out using multiplex-polymerase chain reaction (PCR). Results: Of 120 samples, 60 (50%) instances of K. pneumoniae were isolated. The highest and lowest resistance rates were related to cefotaxime (CTX) (50%) and ciprofloxacin (CIP) (23.3%), respectively. 18.3% (n: 11) K. pneumoniae showed an AmpC phenotype in DCDT and TDET tests. AmpC genotyping showed that the prevalence of CIT, DHA, EBC, and ACC genotypes were 47.1%, 35.3%, 29.5%, and 23.6%, respectively. MOX and FOX genotypes were not identified in the isolates of interest. Conclusion: AmpC ?-lactamase identification tests should be considered a routine microbiology workup for gram-negative microorganisms. Multiplex-PCR can identify the plasmid AmpC genotypes. ? | en |
| DOI | https://doi.org/10.22037/jcma.v6i3.35261 | en |
| Keyword | AmpC | en |
| Keyword | Klebsiella pneumoniae | en |
| Keyword | double-disc plus combined test | en |
| Keyword | three-dimensional extract test. | en |
| Publisher | Brieflands | en |
| Title | Molecular Epidemiology of AmpC-Producing Klebsiella Pneumoniae Isolated from Perioperative Patients in a Tertiary Hospital | en |
| Type | Original Articles | en |
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