Effect of Eicosapentaenoic Acid on <i>sFlt-1</i> and HIF-1α Expression Under Induced Hypoxia Conditions in Trophoblast Tumor Cell Line (JEG-3)
| Author | Cobra Moradian | en |
| Author | Behrooz Motlagh | en |
| Author | Zahra Afshari | en |
| Orcid | Cobra Moradian [0000-0002-3948-4642] | en |
| Orcid | Behrooz Motlagh [0000-0002-0837-0933] | en |
| Orcid | Zahra Afshari [0000-0002-4515-1158] | en |
| Issued Date | 2021-11-30 | en |
| Abstract | Background: Previous studies have shown the altered levels of long-chain polyunsaturated fatty acids (LCPUFAs) in pathological hypoxic conditions. Elevated soluble fms-like tyrosine kinase-1 (sFlt-1) expression in hypoxia plays an important role in the pathogenesis of placental as preeclampsia. Objectives: The eicosapentaenoic acid (EPA; 20:5, n-3) as LCPUFAs (omega-3) might attenuate sFlt-1 and hypoxia-inducible factor-1α (HIF-1α) expressions and secretions. Methods: JEG-3 cells were incubated with dimethyloxalylglycine (DMOG) and EPA. The SFlt-1 gene expression was determined using a real-time polymerase chain reaction. The protein secretion of sFlt-1 and HIF-1α were analyzed using Western blot. Results: The incubation of JEG-3 cells with DMOG significantly elevated messenger ribonucleic acid (mRNA) expression and protein secretion of sFlt-1 (P < 0.05); nevertheless, EPA decreased mRNA expression and protein secretion of sFlt-1 (P < 0.05). Moreover, EPA inhibited the effect of DMOG on sFlt-1 (P = 0.0361) gene expression and protein secretion and HIF-1α (P = 0.0241) protein secretion. Conclusions: The sFlt-1 expression decreased by n-3 fatty acids in trophoblast tumor cell line under induced hypoxia conditions. It seems that changes in sFlt-1 expression are mediated by the transcription factor HIF-1α. | en |
| DOI | https://doi.org/10.5812/tms-122263 | en |
| Keyword | EPA | en |
| Keyword | HIF-1α | en |
| Keyword | Preeclampsia | en |
| Keyword | Hypoxia | en |
| Keyword | <i>sFlt-1</i> | en |
| Publisher | Brieflands | en |
| Title | Effect of Eicosapentaenoic Acid on <i>sFlt-1</i> and HIF-1α Expression Under Induced Hypoxia Conditions in Trophoblast Tumor Cell Line (JEG-3) | en |
| Type | Research Article | en |