Evaluation of NLR Family CARD Domain Containing 3 and NLR Family CARD Domain Containing 5 Gene Expression in Interferon Gamma–Treated Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
| Author | Aida Nahumi | en |
| Author | Leila Pirdel | en |
| Author | Asadollah Asadi | en |
| Author | Arash Abdolmaleki | en |
| Orcid | Aida Nahumi [0000-0002-1781-0224] | en |
| Orcid | Asadollah Asadi [0000-0003-3314-2948] | en |
| Orcid | Arash Abdolmaleki [0000-0002-7454-8728] | en |
| Issued Date | 2022-04-30 | en |
| Abstract | Background: Human umbilical cord Wharton’s jelly has provided a new source for mesenchymal stem cells (MSCs). The highly proliferative capacity with low immunogenicity and multi-differentiation potential of its stem cells make them applicable for transplantation purposes. Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play various roles in antigen presentation of pathogens and damaged cells to suppress and/or modulate inflammation. Objectives: In this study, the expression levels of NLR family CARD domain containing 3 (NLRC3) and NLRC5 genes were analyzed and compared in both untreated and interferon gamma (IFN-γ)–treated Wharton’s jelly-derived MSCs (WJ-MSCs). Methods: MSCs were isolated from human umbilical cord Wharton’s jelly using standard tissue culture. The expression of NLRC5 and NLRC3 genes was analyzed in IFN-γ–treated WJ-MSCs (24 hours after treatment) and untreated WJ-MSCs (as a control) using quantitative real-time polymerase chain reaction (PCR). Results: It was found that IFN-γ treatment mimicking an inflammation scenario led to a statistically significant increase of NLRC3 and NLRC5 gene expression compared to untreated WJ-MSCs (P ≤ 0.05). Conclusions: It seems that higher expression of NLRC3 and NLRC5 genes in treated WJ-MSCs may make them a proper candidate to be used as a source for cell therapy in inflammatory conditions. | en |
| DOI | https://doi.org/10.5812/gct.118882 | en |
| Keyword | Cytokine | en |
| Keyword | Gene Expression | en |
| Keyword | Immunomodulation | en |
| Keyword | Mesenchymal Stem Cells | en |
| Keyword | NOD-Like Receptors | en |
| Publisher | Brieflands | en |
| Title | Evaluation of NLR Family CARD Domain Containing 3 and NLR Family CARD Domain Containing 5 Gene Expression in Interferon Gamma–Treated Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord | en |
| Type | Research Article | en |